p53 (pSer392) antibody

Rabbit anti p53 (pSer392)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
p53 (pSer392)

Product Code Applications Pack Size List Price Your Price Qty
AHP906
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
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loader
Search for Batch Specific Datasheets

Rabbit anti p53 (pSer392) antibody recognizes cellular tumor antigen p53, also known as antigen NY-CO-13, phosphoprotein p53 and tumor suppressor p53, when phosphorylated at serine 392.

The tumor suppressor protein p53, is expressed ubiquitously and plays an important role in blocking proliferation of damaged cells.

Phosphorylation of p53 at serine 392 is reported in response to diverse stresses, for example UV irradiation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Monkey Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human phosphorylated p53 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within p53 which includes the phosphorylated residue serine 392.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP906 recognises a band of approximately 53kD in rat brain nuclear fraction lysates.

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References for p53 (pSer392) antibody

  1. Jaiswal, A.S. & Narayan, S. (2001) p53-dependent transcriptional regulation of the APC promoter in colon cancer cells treated with DNA alkylating agents.
    J Biol Chem. 276 (21): 18193-9.

RRID
AB_567186
UniProt
P04637
Entrez Gene
TP53
View more products with P53 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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