p53 (pSer15) antibody

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Rabbit anti p53 (pSer15)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2339 IP P WB* 50 µg
Rabbit anti p53 (pSer15) antibody recognizes cellular tumor antigen p53, also known as antigen NY-CO-13, phosphoprotein p53 and tumor suppressor p53, when phosphorylated at serine 15.

The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor, involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007).
The Rabbit anti p53 (pSer 15) antibody specifically binds to p53 phosphorylated on serine 15 in response to DNA damage. The kinases ATM, ATR, and DNA-PK are all able to phosphorylate p53 on serine 15, which impairs its capacity to bind to the E3 ubiquitin protein ligase MDM2.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic phospho-peptide surrounding amino acid Ser15 of human p53
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P04637 Related reagents
Entrez Gene
TP53 Related reagents
Regulatory
For research purposes only

Applications of p53 (pSer15) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 20 ug/ml
Immunoprecipitation 5 10 ug/ml
Western Blotting 1 0.5 4.0 ug/ml
  1. 1For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti p53 (pSer15) antibody recognizes a band of approximately 53 kDa in lysate from HeLa cells treated with UV

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB