p53 (pSer15) antibody
Rabbit anti p53 (pSer15) antibody recognizes cellular tumor antigen p53, also known as antigen NY-CO-13, phosphoprotein p53 and tumor suppressor p53, when phosphorylated at serine 15.
The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor, involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007).
Rabbit anti p53 (pSer15) antibody binds to p53 phosphorylated on serine 15 in response to DNA damage. The kinases ATM, ATR, and DNA-PK are all able to phosphorylate p53 on serine 15, which impairs its capacity to bind to the E3 ubiquitin protein ligase MDM2.
The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (
Rabbit anti p53 (pSer15) antibody binds to p53 phosphorylated on serine 15 in response to DNA damage. The kinases ATM, ATR, and DNA-PK are all able to phosphorylate p53 on serine 15, which impairs its capacity to bind to the E3 ubiquitin protein ligase MDM2.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal - Immunogen
- Synthetic phospho-peptide surrounding amino acid Ser15 of human p53
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of p53 (pSer15) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 20 ug/ml | ||
| Immunoprecipitation | 5 | 10 ug/ml | |
| Western Blotting | 0.5 | 4.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti p53 (pSer15) antibody recognizes a band of approximately 53 kDa in lysate from HeLa cells treated with UV
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
Further Reading
-
Madan, E. et al. (2012) p53 Ser15 phosphorylation disrupts the p53-RPA70 complex and induces RPA70-mediated DNA repair in hypoxia.
Biochem J. 443 (3): 811-20. -
Wang, B. et al. (2013) p53 Ser15 phosphorylation and histone modifications contribute to IR-induced miR-34a transcription in mammary epithelial cells.
Cell Cycle. 12 (13): 2073-83. -
Yang, T. et al. (2019) Phosphorylation of p53 Serine 15 Is a Predictor of Survival for Patients with Hepatocellular Carcinoma.
Can J Gastroenterol Hepatol. 2019: 9015453.
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