p27/Kip1 antibody

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Rabbit anti p27/Kip1

p27/Kip1 Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00721 WB 100 µl
VPA00721KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human p27/kip1 antibody recognizes the cyclin-dependent kinase inhibitor 1, also known as CDK-interacting protein 1, KIP1, BWS, P27KIP1 or wild-type p53-activated fragment 1.

The CDKN1A gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of CDKN1A is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for CDKN1A (provided by RefSeq, Nov 2010).

Rabbit anti Human p27/kip1 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered glycine
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Recombinant protein encompassing part of the center region of human p27/kip1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P46527 Related reagents
Entrez Gene
CDKN1B Related reagents
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
GO:0005654 nucleoplasm
GO:0006917 induction of apoptosis
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007050 cell cycle arrest
GO:0048102 autophagic cell death
GO:0008285 negative regulation of cell proliferation
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030308 negative regulation of cell growth
GO:0032582 negative regulation of gene-specific transcription
GO:0048011 nerve growth factor receptor signaling pathway
GO:0071285 cellular response to lithium ion
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of p27/Kip1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Rabbit anti p27/kip1 detects a band of approximately 27 kDa in NIH/3T3 cell lysates
Western Blotting
Rabbit anti p27/kip1 detects a band of approximately 27 kDa in NIH/3T3 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB