p27/Kip1 antibody
Aberrant p27Kip1. levels have been reported in breast and colorectal cancers (Sgambato et al. 1997, Ogino et al. 2007).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antiserum to p27/Kip1 was raised by repeated immunisation of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.2% Gelatin - Immunogen
- Peptide mapping to the carboxy terminus of human p27/Kip1
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46527
- Entrez Gene
- CDKN1B
- GO Terms
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005829 cytosol
- GO:0004861 cyclin-dependent protein kinase inhibitor activity
- GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
- GO:0005654 nucleoplasm
- GO:0006917 induction of apoptosis
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0007050 cell cycle arrest
- GO:0048102 autophagic cell death
- GO:0008285 negative regulation of cell proliferation
- GO:0019903 protein phosphatase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030308 negative regulation of cell growth
- GO:0032582 negative regulation of gene-specific transcription
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0071285 cellular response to lithium ion
- Regulatory
- For research purposes only
Applications of p27/Kip1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/200 |
- Western Blotting
- Rabbit anti p27/Kip1 reconizes a band of approximately 27 kDa when run under reducing conditions
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
![]() |
|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
![]() |
|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
![]() |
|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
![]() |
|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra

Mini-review
Understanding the Eukaryotic Cell Cycle - A Biological and Experimental Overview
Read or Download Now