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p27/Kip1 antibody

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Anti p27/Kip1 Antibody gallery image 1
Western blot analysis of (A) A431, (B) HL60 and (C) HeLa whole cell lysates probed with Rabbit anti p27/Kip1

Rabbit anti p27/Kip1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2409 WB 0.1 mg
Rabbit anti p27/Kip1 antibody recognizes cyclin-dependent kinase inhibitor p27 (p27), also known as cyclin-dependent kinase inhibitor 1B or p27Kip1. The protein is a cyclin-dependent kinase inhibitor (CKI) that belongs to the Cip/Kip protein family (Cerqueira et al. 2014). As the name implies, CKIs play a role in the regulation of cyclin-dependent kinases (CDKs) and cyclin-CDK complexes (Cerqueira et al. 2014).

Aberrant p27Kip1. levels have been reported in breast and colorectal cancers (Sgambato et al. 1997, Ogino et al. 2007).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to p27/Kip1 was raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Peptide mapping to the carboxy terminus of human p27/Kip1
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P46527 Related reagents
Entrez Gene
CDKN1B Related reagents
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
GO:0005654 nucleoplasm
GO:0006917 induction of apoptosis
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007050 cell cycle arrest
GO:0048102 autophagic cell death
GO:0008285 negative regulation of cell proliferation
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030308 negative regulation of cell growth
GO:0032582 negative regulation of gene-specific transcription
GO:0048011 nerve growth factor receptor signaling pathway
GO:0071285 cellular response to lithium ion
Regulatory
For research purposes only

Applications of p27/Kip1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti p27/Kip1 reconizes a band of approximately 27 kDa when run under reducing conditions
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
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