Osteopontin antibody

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Goat anti Human Osteopontin (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1069 E WB 0.1 mg
Goat anti Human Osteopontin antibody recognises an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated ~60 kDa glycoprotein. Osteopontin is a secreted chemokine-like protein and member of the SIBLING family, and is synthesised by many cell types.

Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.

Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid, ready to use.
Antiserum Preparation
Antisera to osteopontin (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10451 Related reagents
Entrez Gene
SPP1 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007566 embryo implantation
GO:0031214 biomineral tissue development
GO:0033280 response to vitamin D
GO:0046697 decidualization
Regulatory
For research purposes only

Applications of Osteopontin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/ 64000
Western Blotting 1.0 3.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1069 detects a band of approximately 60kDa in lysates of human thymus, tonsil and spleen. Approximate 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glcoprotein.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for Osteopontin antibody

  1. Rangaswami, H. et al. (2006) Osteopontin: role in cell signaling and cancer progression.
    Trens Cell Biol. 16: 79-87.