Osteopontin antibody

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Anti Human Osteopontin (C-Terminal) Antibody thumbnail image 1

Anti Human Osteopontin (C-Terminal) Antibody gallery image 1 — Western blotting analysis of MOLT4 lysate (35µg protein in RIPA buffer) using Goat anti Human osteopontin antibody (**AHP1069**) at 0.5μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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Goat anti Human Osteopontin (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Osteopontin
Region: (C-TERMINAL)

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Secondary Antibodies
References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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AHP1069 Datasheet Datasheet SDS SDS	E WB	0.1 mg		Log in	Get Quote
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Goat anti Human Osteopontin antibody recognises an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated ~60 kDa glycoprotein. Osteopontin is a secreted chemokine-like protein and member of the SIBLING family, and is synthesised by many cell types.

Osteopontin is involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. It is essential in the pathway that leads to type I immunity. Osteoponitn is also thought to be important to cell-matrix interaction, and can bind fibronectin, collagen and calcium.

Osteopontin has been suggested to have a role in atherosclerosis, bone remodelling, angiogenesis, wound healing and tissue injuries.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Dog Expected from Sequence

Rat Expected from Sequence

Mouse Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid, ready to use.
Antiserum Preparation: Antisera to osteopontin (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin.
Approx. Protein Concentrations: IgG concentration 0.5mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Dog	Expected from Sequence
Rat	Expected from Sequence
Mouse	Expected from Sequence

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/ 64000
Western Blotting		1.0	3.0 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1069 detects a band of approximately 60kDa in lysates of human thymus, tonsil and spleen. Approximate 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glcoprotein.

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Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in	Get Quote
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Description	Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in	Get Quote
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Description	Rabbit anti Goat IgG (Fc):HRP

References for Osteopontin antibody

Rangaswami, H. et al. (2006) Osteopontin: role in cell signaling and cancer progression.
Trens Cell Biol. 16: 79-87.

Synonyms: SPP1
RRID: AB_2194836
UniProt: P10451
Entrez Gene: SPP1
GO Terms: GO:0007155 cell adhesion; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0007566 embryo implantation; GO:0031214 biomineral tissue development; GO:0033280 response to vitamin D; GO:0046697 decidualization