Osteocalcin antibody

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Rabbit anti Human Osteocalcin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7060-1515 C 0.25 ml
Rabbit anti Human osteocalcin antibody detects human osteocalcin and recognizes an epitope towards the C-terminal of the molecule.

Osteocalcin (Bone Gla Protein) is a 49 amino acid single chain vitamin K dependent protein (MW 5.8 kDa), made by osteoblasts that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget's disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Chicken
Rat
Bovine
Monkey
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Native human osteocalcin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02818 Related reagents
Entrez Gene
BGLAP Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0007155 cell adhesion
GO:0008147 structural constituent of bone
GO:0030282 bone mineralization
GO:0030500 regulation of bone mineralization
GO:0042476 odontogenesis
GO:0045124 regulation of bone resorption
GO:0045670 regulation of osteoclast differentiation
GO:0046848 hydroxyapatite binding
Regulatory
For research purposes only

Applications of Osteocalcin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
7060-1515 is suitable for staining cultured human osteoblasts after fixation with 2% formaldehyde and permeabilization.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for Osteocalcin antibody

  1. Bielby RC et al. (2004) In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells.
    Tissue Eng. 10 (9-10): 1518-25.
  2. Lopes, M.A. et al. (1998) Flow cytometry for assessing biocompatibility.
    J Biomed Mater Res. 41 (4): 649-56.
  3. Kaivosoja, E. et al. (2015) The effect of pulsed electromagnetic fields and dehydroepiandrosterone on viability and osteo-induction of human mesenchymal stem cells.
    J Tissue Eng Regen Med. 9 (1): 31-40.
  4. Myllymaa, S. et al. (2010) Adhesion, spreading and osteogenic differentiation of mesenchymal stem cells cultured on micropatterned amorphous diamond, titanium, tantalum and chromium coatings on silicon.
    J Mater Sci Mater Med. 21 (1): 329-41.