op18 antibody

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Rabbit anti op18 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1493 P* WB 0.1 mg
Rabbit anti Human op18 antibody recognises the C-terminal regionHuman op18, a 17.3 kDa cytoplasmic protein belonging to the Strathmin family.

Op18 may function as an intracellular relay by integrating several signalling pathways such as those involved in cell proliferation and differentiation. In addition, op18 is involved in the regulation of the microtubule filament system by destabilizing microtubules where it prevents assembly, and promotes disassembly of microtubules.

op18 is a potential target of the ASK1-p38 MAP kinase cascade suggesting that the ASK1-p38 cascade may regulate microtubule dynmamics through op18.

Op18, while ubiquitously expressed in normal tissues, is highly expressed in a variety of cancers including leukaemia, prostate cancer, ovarian carcinoma and breast carcinoma. It has therefore been hypothesized that op18 may be a potential target for anti-cancer therapeutics.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human op18 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 20 amino acid peptide located near the carboxy terminus of human op18.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16949 Related reagents
Entrez Gene
STMN1 Related reagents
GO Terms
GO:0009615 response to virus
GO:0004871 signal transducer activity
GO:0035556 intracellular signal transduction
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0007019 microtubule depolymerization
GO:0007052 mitotic spindle organization
GO:0007399 nervous system development
GO:0015631 tubulin binding
GO:0030154 cell differentiation
Regulatory
For research purposes only

Applications of op18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1493 detects a band of approximately 15kDa in EL4 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Mizumura, K. et al. (2006) Identification of Op18/stathmin as a potential target of ASK1-p38 MAP kinase cascade.
    J Cell Physiol. 206 (2): 363-70.
  2. Mistry, S.J. & Atweh, G.F. (2002) Role of stathmin in the regulation of the mitotic spindle: potential applications in cancer therapy.
    Mt Sinai J Med. 69 (5): 299-304.
  3. Curmi, P.A. et al. (1999) Stathmin and its phosphoprotein family: general properties, biochemical and functional interaction with tubulin.
    Cell Struct Funct. 24 (5): 345-57.
  4. Belmont, L.D. & Mitchison, T.J. (1996) Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
    Cell. 84 (4): 623-31.