NUP62 antibody
Rabbit anti Human NUP62 antibody recognizes the nuclear pore glycoprotein p62, also known as 62 kDa nucleoporin or nucleoporin Nup62.
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by NUP62 gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of NUP62 encode a single protein isoform (provided by RefSeq, Jul 2008).
Rabbit anti Human NUP62 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by NUP62 gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of NUP62 encode a single protein isoform (provided by RefSeq, Jul 2008).
Rabbit anti Human NUP62 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti NUP62 detects a band of approximately 72 kDa in MOLT-4 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered glycine
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Preservative Stabilisers
- 0.01% Thiomersal
<50% Glycerol - Immunogen
- Recombinant protein encompassing part of the middle region of human NUP62
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MOLT-4 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P37198 Related reagents
- Entrez Gene
- NUP62 Related reagents
- GO Terms
- GO:0000922 spindle pole
- GO:0003682 chromatin binding
- GO:0030529 ribonucleoprotein complex
- GO:0004674 protein serine/threonine kinase activity
- GO:0005643 nuclear pore
- GO:0005737 cytoplasm
- GO:0005975 carbohydrate metabolic process
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008219 cell death
- GO:0008285 negative regulation of cell proliferation
- GO:0016032 viral reproduction
- GO:0009755 hormone-mediated signaling pathway
- GO:0010827 regulation of glucose transport
- GO:0015031 protein transport
- GO:0015758 glucose transport
- GO:0046966 thyroid hormone receptor binding
- GO:0017056 structural constituent of nuclear pore
- GO:0030159 receptor signaling complex scaffold activity
- GO:0030528 transcription regulator activity
- GO:0031074 nucleocytoplasmic shuttling complex
- GO:0031965 nuclear membrane
- GO:0042169 SH2 domain binding
- GO:0043066 negative regulation of apoptosis
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043130 ubiquitin binding
- GO:0045742 positive regulation of epidermal growth factor receptor signaling pathway
- GO:0045941 positive regulation of transcription
- GO:0051028 mRNA transport
- GO:0055085 transmembrane transport
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NUP62 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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