NSFL1C antibody

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Anti NSFL1C Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with NSFL1C antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 36 second exposure. Arrow points to NSFL1C (molecular weight 41 kDa).

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Rabbit anti NSFL1C

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: NSFL1C

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VPA00767 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human NSFL1C antibody recognizes NSFL1 cofactor p47, also known as SHP1 homolog, UBX domain-containing protein 2C or p97 cofactor p47.

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by NSFL1C binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8 (provided by RefSeq, May 2011).

Rabbit anti Human NSFL1C antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti NSFL1C antibody recognizes a band of approximately 41 kDa in A431 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between amino acids 214-248 of human NSFL1C
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q9UNZ2
Entrez Gene: NSFL1C
GO Terms: GO:0005515 protein binding; GO:0005634 nucleus; GO:0005694 chromosome; GO:0005795 Golgi stack; GO:0008289 lipid binding