NPM antibody
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WB ResetGoat anti NPM
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- NPM
Antibody Quality and Performance Guarantee
| Goat anti Human NPM antibody recognizes NPM, also known as nucleophosmin. NPM is a major nucleolar phosphoprotein expressed at notably higher levels in proliferating and tumor cells, compared to resting cells. It plays important roles in ribosome biogenesis, centrosome duplication and cell proliferation. NPM is a mobile protein which shuttles between the nucleolus and cytoplasm depending upon growth conditions and ATP/GTP levels, and is also influenced by cytotoxic drugs including actinomycin D and camptothecin. NPM interacts with numerous proteins including p19ARF, transcription factor YY1 and nucleolar p120, and is known to regulate the stability and transcriptional activity of p53, a tumor suppressor protein activated in response to DNA damage, capable of inducing cell cycle arrest and apoptosis. Goat anti Human NPM antibody detects nucleophosmin as a single band of 38 kDa in a range of human cell lysates tested. |
|
- Target Species
- Human
- Western Blotting
- Goat anti NPM detects a band of approximately 38 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-QEAIQDLWQWRKSL from the C terminus of the protein sequence.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
VPA00058
091014
166728
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