NPM antibody

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Goat anti NPM

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00058
Datasheet
WB
MSDS
100 µl

Goat anti Human NPM antibody recognizes NPM, also known as nucleophosmin. NPM is a major nucleolar phosphoprotein expressed at notably higher levels in proliferating and tumor cells, compared to resting cells. It plays important roles in ribosome biogenesis, centrosome duplication and cell proliferation.

NPM is a mobile protein which shuttles between the nucleolus and cytoplasm depending upon growth conditions and ATP/GTP levels, and is also influenced by cytotoxic drugs including actinomycin D and camptothecin. NPM interacts with numerous proteins including p19ARF, transcription factor YY1 and nucleolar p120, and is known to regulate the stability and transcriptional activity of p53, a tumor suppressor protein activated in response to DNA damage, capable of inducing cell cycle arrest and apoptosis.

Goat anti Human NPM antibody detects nucleophosmin as a single band of 38 kDa in a range of human cell lysates tested.

Product Details

Target Species
Human
Western Blotting
Goat anti NPM detects a band of approximately 38 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-QEAIQDLWQWRKSL from the C terminus of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P06748
Entrez Gene
NPM1
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NPM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader