NPM antibody

Goat anti NPM

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
NPM

Product Code Applications Pack Size List Price Your Price Qty
VPA00058
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human NPM antibody recognizes NPM, also known as nucleophosmin. NPM is a major nucleolar phosphoprotein expressed at notably higher levels in proliferating and tumor cells, compared to resting cells. It plays important roles in ribosome biogenesis, centrosome duplication and cell proliferation.

NPM is a mobile protein which shuttles between the nucleolus and cytoplasm depending upon growth conditions and ATP/GTP levels, and is also influenced by cytotoxic drugs including actinomycin D and camptothecin. NPM interacts with numerous proteins including p19ARF, transcription factor YY1 and nucleolar p120, and is known to regulate the stability and transcriptional activity of p53, a tumor suppressor protein activated in response to DNA damage, capable of inducing cell cycle arrest and apoptosis.

Goat anti Human NPM antibody detects nucleophosmin as a single band of 38 kDa in a range of human cell lysates tested.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Goat anti NPM detects a band of approximately 38 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-QEAIQDLWQWRKSL from the C terminus of the protein sequence.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Synonyms
Nucleophosmin
UniProt
P06748
Entrez Gene
NPM1
View more products with NPM specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products