NOXO1 antibody
Rabbit anti Human NOXO1 antibody recognizes human NADPH oxidase organizer 1, also known as NOXO1, NADPH oxidase regulatory protein, Nox organizer 1, Nox-organizing protein 1 or SH3 and PX domain-containing protein 5. NOXO1 is a 376 amino acid ~48 kDa cell membrane associated protein involved in the superoxide-generating activity of NOX1 and NOX3 and is required for the development of crystalline gravity sensing structures of the inner ear (Lundberg et al. 2015).
Multiple isoforms of human NOXO1 generated by alternative splicing have been described. Rabbit anti Human NOXO1 antibody recognizes an epitope within the sequence “SGPQFCASRAYES” representing amino acids 238-252 of isoform 1 of human NOXO1, this sequence is common to all described isoforms of human NOXO1 (UniProt : Q8NFA2).
Multiple isoforms of human NOXO1 generated by alternative splicing have been described. Rabbit anti Human NOXO1 antibody recognizes an epitope within the sequence “SGPQFCASRAYES” representing amino acids 238-252 of isoform 1 of human NOXO1, this sequence is common to all described isoforms of human NOXO1 (UniProt : Q8NFA2).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human NOXO1was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Immunogen
- Recombinant Human NOXO1.
- Approx. Protein Concentrations
- Total protein concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8NFA2
- Entrez Gene
- NOXO1
- GO Terms
- GO:0007154 cell communication
- GO:0010310 regulation of hydrogen peroxide metabolic process
- GO:0016176 superoxide-generating NADPH oxidase activator activity
- GO:0019899 enzyme binding
- GO:0035091 phosphatidylinositol binding
- GO:0043020 NADPH oxidase complex
- GO:0060263 regulation of respiratory burst
- Regulatory
- For research purposes only
Applications of NOXO1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1:20000 | 1:100000 | |
| Immunofluorescence | |||
| Immunohistology - Paraffin | 5 | 10ug/ml | |
| Western Blotting | 1:2000 | 1:8000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Human colon
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| Histar Detection System | STAR3000A | C P | 50 Tests |
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