NOXO1 antibody

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Rabbit anti Human NOXO1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2050	E IF P WB			50 µg

Rabbit anti Human NOXO1 antibody recognizes human NADPH oxidase organizer 1, also known as NOXO1, NADPH oxidase regulatory protein, Nox organizer 1, Nox-organizing protein 1 or SH3 and PX domain-containing protein 5. NOXO1 is a 376 amino acid ~48 kDa cell membrane associated protein involved in the superoxide-generating activity of NOX1 and NOX3 and is required for the development of crystalline gravity sensing structures of the inner ear (Lundberg et al. 2015).

Multiple isoforms of human NOXO1 generated by alternative splicing have been described. Rabbit anti Human NOXO1 antibody recognizes an epitope within the sequence “SGPQFCASRAYES” representing amino acids 238-252 of isoform 1 of human NOXO1, this sequence is common to all described isoforms of human NOXO1 (UniProt : Q8NFA2).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Chimpanzee	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human NOXO1was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01% Sodium Azide (NaN₃)

Immunogen

Recombinant Human NOXO1.

Approx. Protein Concentrations

Total protein concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q8NFA2 Related reagents
Entrez Gene: NOXO1 Related reagents
GO Terms: GO:0007154 cell communication; GO:0010310 regulation of hydrogen peroxide metabolic process; GO:0016176 superoxide-generating NADPH oxidase activator activity; GO:0019899 enzyme binding; GO:0035091 phosphatidylinositol binding; GO:0043020 NADPH oxidase complex; GO:0060263 regulation of respiratory burst
Regulatory: For research purposes only

Applications of NOXO1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1:20000	1:100000
Immunofluorescence
Immunohistology - Paraffin	5	10ug/ml
Western Blotting	1:2000	1:8000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue: Human colon

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
Histar Detection System	STAR3000A	50 Tests	C P
Histar Detection System	STAR3000B	150 Tests	C P
Histar Detection System	STAR3000C	500 Tests	C P

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