Noxa antibody

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Goat anti Human Noxa

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2170 E WB 0.1 mg
Goat anti Human Noxa antibody recognizes human Noxa, a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation.

Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human NOXA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-TQLRRFGDKLNFRQK from the internal region of Noxa (NP_066950.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q13794 Related reagents
Entrez Gene
PMAIP1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0001836 release of cytochrome c from mitochondria
GO:0005829 cytosol
GO:0005741 mitochondrial outer membrane
GO:0006919 activation of caspase activity
GO:0006921 cellular component disassembly involved in apoptosis
GO:0006926 virus-infected cell apoptosis
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0043331 response to dsRNA
Regulatory
For research purposes only

Applications of Noxa antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 0.03 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2170 detects a band of approximately 16kDa in human cerebellum cell lysates.
Suitable loading control:
Goat anti Human GAPDH; product code(AHP1064)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for Noxa antibody

  1. Yu, S.J. et al. (2013) MicroRNA-200a promotes anoikis resistance and metastasis by targeting YAP1 in human breast cancer.
    Clin Cancer Res. 19 (6): 1389-99.
  2. Ikezoe, T. et al. (2010) p53 is critical for the Aurora B kinase inhibitor-mediated apoptosis in acute myelogenous leukemia cells.
    Int J Hematol. 91: 69-77.
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