NonO antibody
Goat anti Human NonO antibody recognizes the NonO protein, also known as non-POU domain-containing octamer-binding protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55 and p54(nrb).
Encoded by the NONO gene, NonO is an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between NONO gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16 (provided by RefSeq, Feb 2009).
Goat anti Human NonO antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the NONO gene, NonO is an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between NONO gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16 (provided by RefSeq, Feb 2009).
Goat anti Human NonO antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NonO detects a band of approximately 55 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C_NRAAPGAEFAPNK, from the C Terminus of the protein sequence.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q15233
- Entrez Gene
- NONO
- GO Terms
- GO:0000166 nucleotide binding
- GO:0003677 DNA binding
- GO:0003723 RNA binding
- GO:0006281 DNA repair
- GO:0006310 DNA recombination
- GO:0006350 transcription
- GO:0006397 mRNA processing
- GO:0008380 RNA splicing
- GO:0042802 identical protein binding
- GO:0016363 nuclear matrix
- GO:0042382 paraspeckles
- GO:0045449 regulation of transcription
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of NonO antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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