NonO antibody

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Anti NonO Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human NonO antibody (**VPA00108**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to NonO (molecular weight 55 kDa).

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Goat anti NonO

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: NonO

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00108 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human NonO antibody recognizes the NonO protein, also known as non-POU domain-containing octamer-binding protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, NMT55 and p54(nrb).

Encoded by the NONO gene, NonO is an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between NONO gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16 (provided by RefSeq, Feb 2009).

Goat anti Human NonO antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti NonO detects a band of approximately 55 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C_NRAAPGAEFAPNK, from the C Terminus of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: Q15233
Entrez Gene: NONO
GO Terms: GO:0000166 nucleotide binding; GO:0003677 DNA binding; GO:0003723 RNA binding; GO:0006281 DNA repair; GO:0006310 DNA recombination; GO:0006350 transcription; GO:0006397 mRNA processing; GO:0008380 RNA splicing; GO:0042802 identical protein binding; View More GO Terms; GO:0016363 nuclear matrix; GO:0042382 paraspeckles; GO:0045449 regulation of transcription