Non-Neuronal Enolase antibody
Rabbit anti Non-Neuronal Enolase
Non-neuronal enolase antibody recognizes alpha-enolase, also known as 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, enolase 1, phosphopyruvate hydratase or plasminogen-binding protein. The antibody has been extensively validated for western blotting using whole cell lysates.
Non-Neuronal Enolase Antibody with Control Lysate
Non-neuronal enolase antibody recognizes alpha-enolase, also known as 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, enolase 1, MBP-1, MPB-1, non-neural enolase, NNE, phosphopyruvate hydratase or plasminogen-binding protein.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00075 | WB | 100 µl | ||||
| VPA00075KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human non-neuronal enolase antibody recognizes human non-neuronal enolase (NNE), also known as alpha enolase or enolase 1.
Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. Enolase is a glycolytic enzyme that catalyzes the dehydratation of 2-phosphoglycerate to phosphoenolpyruvate, in the last steps of the catabolic glycolytic pathway.
Rabbit anti Human non-neuronal enolase antibody detects NNE as a single band of 47 kDa in a wide range of human cell line lysates. Reactivity with the mouse NIH/3T3 fibroblast cell line is also observed.
Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. Enolase is a glycolytic enzyme that catalyzes the dehydratation of 2-phosphoglycerate to phosphoenolpyruvate, in the last steps of the catabolic glycolytic pathway.
Rabbit anti Human non-neuronal enolase antibody detects NNE as a single band of 47 kDa in a wide range of human cell line lysates. Reactivity with the mouse NIH/3T3 fibroblast cell line is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti non-neuronal enolase detects a band of approximately 47 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native human NNE
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P06733 Related reagents
- Entrez Gene
- ENO1 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Non-Neuronal Enolase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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