NOLC1 antibody
Goat anti NOLC1
Goat anti human NOLC1 antibody recognizes nucleolar and coiled-body phosphoprotein 1, also known as NOPP130, NOPP140, NS5ATP13, 140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13 or nucleolar protein p130. This antibody has been extensively validated for western blotting.
NOLC1 Antibody with Control Lysate
Goat anti human NOLC1 antibody recognizes nucleolar and coiled-body phosphoprotein 1, also known as NOPP130, NOPP140, NS5ATP13, 140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13 or nucleolar protein p130.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00418 | WB | 100 µl | ||||
| VPA00418KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human NOLC1 antibody recognizes NOLC1, also known as nucleolar and coiled-body phosphoprotein 1, HCV NS5A-transactivated protein 13 and nucleolar protein p130.
Goat anti Human NOLC1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Goat anti Human NOLC1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NOLC1 detects a band of approximately 130 kDa in Jurkat cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Peptide sequence C-QVNSIKFDSE
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q14978 Related reagents
- Entrez Gene
- NOLC1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005524 ATP binding
- GO:0005525 GTP binding
- GO:0005737 cytoplasm
- GO:0005730 nucleolus
- GO:0006364 rRNA processing
- GO:0007067 mitosis
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NOLC1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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