Sheep anti Human NFkB p65 antibody
recognizes human full length human p65 (NF-kB), also known as Transcription factor p65 or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. Human NFkB p65 is a 551 amino acid pleiotropic transcription factor containing a single RHD domain
. Sheep anti Human NFkB p65 antibody will also detect proteolytic fragments of NFkB p65 containing the DNA binding RHD domain located in the amino-terminal region. Multiple isoforms of NFkB p65 are generated by alternative splicing. Sheep anti Human NFkB p65 antibody binds to isoforms 1 and 2 containing the intact RHD domain, but it is unclear whether it will bind to isoforms 3 and 4 which have partial differing deletions within the RHD domain.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human NF-kB were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|0.5%||Bovine Serum Albumin|
- Human p65 (residues 12-317) expressed in E. coli
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific enhancer binding RNA polymerase II transcription factor activity
transcription factor complex
cellular defense response
Toll signaling pathway
identical protein binding
response to UV-B
transcription activator activity
transcription repressor activity
cytokine-mediated signaling pathway
protein kinase binding
membrane protein intracellular domain proteolysis
ubiquitin protein ligase binding
negative regulation of gene-specific transcription
activating transcription factor binding
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB cascade
interspecies interaction between organisms
innate immune response
positive regulation of transcription from RNA polymerase II promoter
protein N-terminus binding
nerve growth factor receptor signaling pathway
regulation of inflammatory response
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
defense response to virus
nucleotide-binding oligomerization domain containing 2 signaling pathway
repressing transcription factor binding
response to interleukin-1
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NFkB p65 antibody
|Gel Super Shift Assays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for NFkB p65 antibody
Lee, J.H. et al. (2007) Heparin inhibits NF-κB activationand increases cell death in cerebral endothelial cells after oxygen-glucose deprivation.
J Mol Neurosci. 32: 145-54.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra