NFkB p65 antibody
Rabbit anti NFkB p65
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- NFkB p65
Rabbit anti Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFκB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFκB p65 is a 551 amino acid ~65 kDa subunit of the NFκB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFκB appears to be the p65-p50 heterodimer. In the active form NFκB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NFκB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IκB (UniProt: Q04206) In a gel supershift assay Rabbit anti Human NFκB p65 antibody was found to be active against all p65 containing human, mouse or rat NFκB complexes using 0.5ul to 1.0ul per assay. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to NFkB p65 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
0.01% Sodium Azide - Immunogen
- NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/5000 | 1/25000 | |
Gel Super Shift Assays | 0.5 | 1.0 ul per test | |
Immunofluorescence | |||
Immunoprecipitation | |||
Western Blotting | 1/2000 | 1/5000 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
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Description | Sheep anti Rabbit IgG:RPE |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
References for NFkB p65 antibody
-
Cruz, M.T. et al. (1999) Involvement of JAK2 and MAPK on type II nitric oxide synthase expression in skin-derived dendritic cells.
Am J Physiol. 277: C1050-7. -
Parmentier. M, et al. (2000) Regulation of lipopolysaccharide-mediated interleukin-1beta release by N-acetylcysteine in THP-1 cells.
Eur. Respir. J. 16: 933-9. -
Gründker, C. et al. (2000) Luteinizing hormone-releasing hormone induces nuclear factor kappaB-activation and inhibits apoptosis in ovarian cancer cells.
J Clin Endocrinol Metab. 85: 3815-20. -
Sun, J. et al. (2007) Neuropeptide substance P upregulates chemokine and chemokine receptor expression in primary mouse neutrophils.
Am J Physiol Cell Physiol. 293: C696-704. -
Zhang, H. et al. (2007) Hydrogen sulfide acts as an inflammatory mediator in cecal ligation and puncture-induced sepsis in mice by upregulating the production of cytokines and chemokines via NF-kappaB.
Am J Physiol Lung Cell Mol Physiol. 292 (4): L960-71. -
Takada, Y. et al. (2009) Odoroside A and ouabain inhibit Na+/K+-ATPase and prevent NF-kappaB-inducible protein expression by blocking Na+-dependent amino acid transport.
Biochem Pharmacol. 78 (9): 1157-66. -
Wang, J. et al. (2014) TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.
BMC Nephrol. 15: 178. -
Abdel-Latif, M. (2015) Diethylcarbamazine citrate ameliorates insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation.
Int Immunopharmacol. 29 (2): 607-12.
View The Latest Product References
- RRID
- AB_322343
- UniProt
- Q04206
- Entrez Gene
- RELA
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003682 chromatin binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0006968 cellular defense response
- View More GO Terms
- GO:0008063 Toll signaling pathway
- GO:0042802 identical protein binding
- GO:0010224 response to UV-B
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0019221 cytokine-mediated signaling pathway
- GO:0019901 protein kinase binding
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0031625 ubiquitin protein ligase binding
- GO:0032582 negative regulation of gene-specific transcription
- GO:0033613 activating transcription factor binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042301 phosphate binding
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0047485 protein N-terminus binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050727 regulation of inflammatory response
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051607 defense response to virus
- GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
- GO:0070491 repressing transcription factor binding
- GO:0070555 response to interleukin-1
AHP288
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