NFkB p50 antibody
Rabbit anti NFkB p50 antibody recognizes Nuclear factor NF-kappa-B p50 subunit. NF-kappa-B p105 is a large precursor protein that is cleaved at its C-terminus (which mainly contains ankyrin repeats) by the ubiquitin/proteasome pathway into the NF-kappa-B p50 subunit. The NF-kapp- B p50 subunit is also known as NF-kappa-B1 and interacts with members of the Rel protein family to form transcription factors. In the absence of stimulation, an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.
The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
0.01% thiomersal - Immunogen
- Synthetic peptide corresponding to the N-terminus of human NFkB p50.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19838
- Entrez Gene
- NFKB1
- GO Terms
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006915 apoptosis
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0008063 Toll signaling pathway
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0010843 promoter binding
- GO:0010884 positive regulation of lipid storage
- GO:0010956 negative regulation of calcidiol 1-monooxygenase activity
- GO:0010957 negative regulation of vitamin D biosynthetic process
- GO:0016563 transcription activator activity
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0032269 negative regulation of cellular protein metabolic process
- GO:0032375 negative regulation of cholesterol transport
- GO:0033256 I-kappaB/NF-kappaB complex
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
- Regulatory
- For research purposes only
Applications of NFkB p50 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 0.5 | 4.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti NFkB (p50) antibody recognizes a band of approximately 50 kDa in 3T3 cell lysate and rat kidney tissue lysate
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Secondary Antibodies Available
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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