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NFkB p50 antibody

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Anti NFkB p50 Antibody gallery image 1
Western blot analysis of 3T3 cell lysate (lane 1) and rat kidney tissue lysate (lane 2) probed with Rabbit anti NFkB p50 antibody

Rabbit anti NFkB p50

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2331 WB 50 µg
Rabbit anti NFkB p50 antibody recognizes Nuclear factor NF-kappa-B p50 subunit. NF-kappa-B p105 is a large precursor protein that is cleaved at its C-terminus (which mainly contains ankyrin repeats) by the ubiquitin/proteasome pathway into the NF-kappa-B p50 subunit. The NF-kapp- B p50 subunit is also known as NF-kappa-B1 and interacts with members of the Rel protein family to form transcription factors. In the absence of stimulation, an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.
The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% thiomersal
Immunogen
Synthetic peptide corresponding to the N-terminus of human NFkB p50.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P19838 Related reagents
Entrez Gene
NFKB1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006915 apoptosis
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006366 transcription from RNA polymerase II promoter
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010843 promoter binding
GO:0010884 positive regulation of lipid storage
GO:0010956 negative regulation of calcidiol 1-monooxygenase activity
GO:0010957 negative regulation of vitamin D biosynthetic process
GO:0016563 transcription activator activity
GO:0031293 membrane protein intracellular domain proteolysis
GO:0032269 negative regulation of cellular protein metabolic process
GO:0032375 negative regulation of cholesterol transport
GO:0033256 I-kappaB/NF-kappaB complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of NFkB p50 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti NFkB (p50) antibody recognizes a band of approximately 50 kDa in 3T3 cell lysate and rat kidney tissue lysate
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
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Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB
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