Rabbit anti NFkB p50 antibody recognizes Nuclear factor NF-kappa-B p50 subunit. NF-kappa-B p105 is a large precursor protein that is cleaved at its C-terminus (which mainly contains ankyrin repeats) by the ubiquitin/proteasome pathway into the NF-kappa-B p50 subunit. The NF-kapp- B p50 subunit is also known as NF-kappa-B1 and interacts with members of the Rel protein family to form transcription factors. In the absence of stimulation, an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.
The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
- Synthetic peptide corresponding to the N-terminus of human NFkB p50.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
transcription from RNA polymerase II promoter
Toll signaling pathway
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of macrophage derived foam cell differentiation
positive regulation of lipid storage
negative regulation of calcidiol 1-monooxygenase activity
negative regulation of vitamin D biosynthetic process
transcription activator activity
membrane protein intracellular domain proteolysis
negative regulation of cellular protein metabolic process
negative regulation of cholesterol transport
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
innate immune response
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of canonical Wnt receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NFkB p50 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti NFkB (p50) antibody recognizes a band of approximately 50 kDa in 3T3 cell lysate and rat kidney tissue lysate
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Secondary Antibodies Available