Rabbit anti NFkB p105 (pSer927) antibody
recognizes human nuclear factor NF-kappa-B (NFkB) p105 subunit, also known as DNA-binding factor KBF1, EBP-1 or nuclear factor of kappa light polypeptide gene enhancer in B-cells, when phosphorylated at serine 927.
NFkB p105 is a large precursor protein that is cleaved at its C-terminus (which mainly contains ankyrin repeats) by the ubiquitin/proteasome pathway into the NFkB p50 subunit. The p50 subunit, also known as NFkB1, interacts with members of the Rel protein family to form transcription factors. The NF-kappa-B pathway plays a critical role in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
NFkB p105 is phosphorylated at serine 927 and serine 932 by the IκB kinase (IKK) complex. Phosphorylation at serines 927 and 932 is required for TNF-alpha induced degradation of NFkB p105 (Lang et al. 2003
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to NFkB p105 (pSer927) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 927 of human NFkB p105
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
transcription from RNA polymerase II promoter
Toll signaling pathway
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of macrophage derived foam cell differentiation
positive regulation of lipid storage
negative regulation of calcidiol 1-monooxygenase activity
negative regulation of vitamin D biosynthetic process
transcription activator activity
membrane protein intracellular domain proteolysis
negative regulation of cellular protein metabolic process
negative regulation of cholesterol transport
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
innate immune response
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of canonical Wnt receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NFkB p105 (pSer927) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Lang, V. et al. (2003) betaTrCP-mediated proteolysis of NF-kappaB1 p105 requires phosphorylation of p105 serines 927 and 932.
Mol Cell Biol. 23 (1): 402-13.
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