NFkB p100 (pSer870) antibody
Rabbit anti NFkB p100 (pSer870) antibody recognizes nuclear factor NF-kappa-B p100 subunit (NFkB p100), also known as lymphocyte translocation chromosome 10 protein, nuclear factor Kappa-B subunit 2, nuclear factor of Kappa light chain gene enhancer in B cells 2, oncogene Lyt-10 or transcription factor NFKB2, when phosphorylated at serine 870.
NFkB p100 is a subunit of the NFkB transcription factor complex that is cleaved to form the NFkB p52 subunit. The NFkB pathway plays a critical role in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
Phosphorylation of NFkB p100 at serine 870 is mediated by MAP3K14 (Xiao et al. 2001).
NFkB p100 is a subunit of the NFkB transcription factor complex that is cleaved to form the NFkB p52 subunit. The NFkB pathway plays a critical role in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
Phosphorylation of NFkB p100 at serine 870 is mediated by MAP3K14 (Xiao et al. 2001).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to NFkB p100 (pSer870) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 870 of human NFkB p100
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- Q00653 Related reagents
- Entrez Gene
- NFKB2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0033257 Bcl3/NF-kappaB2 complex
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0045087 innate immune response
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- Regulatory
- For research purposes only
Applications of NFkB p100 (pSer870) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Xiao, G. et al. (2001) NF-kappaB-inducing kinase regulates the processing of NF-kappaB2 p100.
Mol Cell. 7 (2): 401-9.
