NFkB p100 (pSer870) antibody
Rabbit anti NFkB p100 (pSer870) antibody recognizes nuclear factor NF-kappa-B p100 subunit (NFkB p100), also known as lymphocyte translocation chromosome 10 protein, nuclear factor Kappa-B subunit 2, nuclear factor of Kappa light chain gene enhancer in B cells 2, oncogene Lyt-10 or transcription factor NFKB2, when phosphorylated at serine 870.
NFkB p100 is a subunit of the NFkB transcription factor complex that is cleaved to form the NFkB p52 subunit. The NFkB pathway plays a critical role in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
Phosphorylation of NFkB p100 at serine 870 is mediated by MAP3K14 (Xiao et al. 2001).
NFkB p100 is a subunit of the NFkB transcription factor complex that is cleaved to form the NFkB p52 subunit. The NFkB pathway plays a critical role in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
Phosphorylation of NFkB p100 at serine 870 is mediated by MAP3K14 (Xiao et al. 2001).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to NFkB p100 (pSer870) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 870 of human NFkB p100
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q00653
- Entrez Gene
- NFKB2
- GO Terms
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0033257 Bcl3/NF-kappaB2 complex
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0045087 innate immune response
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- Regulatory
- For research purposes only
Applications of NFkB p100 (pSer870) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
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|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Xiao, G. et al. (2001) NF-kappaB-inducing kinase regulates the processing of NF-kappaB2 p100.
Mol Cell. 7 (2): 401-9.
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