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NFkB p100 (pSer870) antibody

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Anti NFkB p100 (pSer870) Antibody gallery image 1
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with TNFα using Rabbit anti NFkB p100 (pSer870) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with TNF-α (20ng/ml) for 30 minutes

Rabbit anti NFkB p100 (pSer870)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2661
Datasheet
WB
MSDS
50 µl loader

Rabbit anti NFkB p100 (pSer870) antibody recognizes nuclear factor NF-kappa-B p100 subunit (NFkB p100), also known as lymphocyte translocation chromosome 10 protein, nuclear factor Kappa-B subunit 2, nuclear factor of Kappa light chain gene enhancer in B cells 2, oncogene Lyt-10 or transcription factor NFKB2, when phosphorylated at serine 870.

NFkB p100 is a subunit of the NFkB transcription factor complex that is cleaved to form the NFkB p52 subunit. The NFkB pathway plays a critical role in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.

Phosphorylation of NFkB p100 at serine 870 is mediated by MAP3K14 (Xiao et al. 2001).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to NFkB p100 (pSer870) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 870 of human NFkB p100

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q00653
Entrez Gene
NFKB2
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0033257 Bcl3/NF-kappaB2 complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Regulatory
For research purposes only

Applications of NFkB p100 (pSer870) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

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Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Xiao, G. et al. (2001) NF-kappaB-inducing kinase regulates the processing of NF-kappaB2 p100.
    Mol Cell. 7 (2): 401-9.
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