Nestin antibody

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Rabbit anti Nestin

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00645 WB 100 µl
Rabbit anti Human nestin antibody recognizes nestin.

The NES gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells (provided by RefSeq, Sep 2011).

Rabbit anti Human nestin antibody detects a band of 290 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti nestin detects a band of approximately 290 kDa in NTERA-2 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human nestin
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P48681 Related reagents
Entrez Gene
NES Related reagents
GO Terms
GO:0005882 intermediate filament
GO:0005198 structural molecule activity
GO:0005737 cytoplasm
GO:0007420 brain development
GO:0019215 intermediate filament binding
GO:0030844 positive regulation of intermediate filament depolymerization
GO:0031076 embryonic camera-type eye development
GO:0043066 negative regulation of apoptosis
GO:2000179 positive regulation of neural precursor cell proliferation
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Nestin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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