NEDD4 antibody
Rabbit anti NEDD4
Rabbit anti-human NEDD4 antibody recognizes the E3 ubiquitin-protein ligase NEDD4, also known as cell proliferation-inducing gene 53 protein, neural precursor cell expressed developmentally down-regulated protein 4 or receptor-potentiating factor 1.
NEDD4 Antibody with Control Lysate
Rabbit anti-human NEDD4 antibody recognizes the E3 ubiquitin-protein ligase NEDD4, also known as cell proliferation-inducing gene 53 protein, neural precursor cell expressed developmentally down-regulated protein 4 or receptor-potentiating factor 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
---|---|---|---|---|---|---|
VPA00462 | WB | 100 µl | ||||
VPA00462KT | WB | 2 Westerns | ||||
With Lysate | 400µg |
Rabbit anti Human NEDD4 antibody detects a band of 115 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NEDD4 detects a band of approximately 115 kDa in NIH/3T3 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid.
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Immunogen
- Synthetic peptide directed towards the middle region of human NEDD4
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg NIH/3T3 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P46934 Related reagents
- Entrez Gene
- NEDD4 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only.
Applications of NEDD4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 | ||
Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
Description | Product Code | Pack Size | Applications | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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