NCF2 antibody
Rabbit anti Human NCF2 antibody recognizes NCF2, also known as 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, chronic granulomatous disease, neutrophil NADPH oxidase factor 2, or neutrophil cytosol factor 2.
NCF2 encodes neutrophil cytosolic factor 2, the 67 kDa cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jun 2010).
Rabbit anti Human NCF2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
NCF2 encodes neutrophil cytosolic factor 2, the 67 kDa cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jun 2010).
Rabbit anti Human NCF2 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NCF2 antibody recognizes a band of approximately 60 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the middle region of human NCF2
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19878
- Entrez Gene
- NCF2
- GO Terms
- GO:0009055 electron carrier activity
- GO:0005737 cytoplasm
- GO:0005730 nucleolus
- GO:0006968 cellular defense response
- GO:0008022 protein C-terminus binding
- GO:0042554 superoxide anion generation
- GO:0043020 NADPH oxidase complex
- GO:0045087 innate immune response
- GO:0045730 respiratory burst
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NCF2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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