NALP3 antibody

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Goat anti Human NALP3 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1774 E P* 50 µg
Goat anti Human NALP3 antibody recognizes human NACHT, LRR and PYD domains-containing protein 3 (NALP3), also known as Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1 or Cryopyrin. NALP3 is a 1036 amino acid ~120kDa cytoplasmic protein belonging to the NLRP family, containing a single DAPIN domain, nine leucine-rich repeats and a single NACHT domain (UniProt: Q96P20) .

NALP3 is involved in inflammation and immune responses. NALP3 may function as an activator of NF-kappaB signalling and play a role in the regulation of inflammation and apoptosis (Choi and Ryter 2014). NALP3 is strongly expressed in polymorphonuclear cells, osteoblasts and chondrocytes, NALP3 can also be detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.

Mutations in the NALP3 gene have been associated with familial cold autoinflammatory syndrome (Hoffman et al. 2001), Muckle-Wells syndrome (Dodé et al. 2002), chronic infantile neurological cutaneous and articular syndrome (Feldmann et al. 2002) and neonatal-onset multisystem inflammatory disease (Aksentijevich et al. 2002).

Goat anti Human NALP3 antibody has been used successfuly for the detection of NALP3 in the eyes of rats by immunohistochemistry on formalin fixed, paraffin embedded sections (Liu et al. 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human NALP3 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence corresponding to C-EKPELTVVFEPSW from the C Terminus of Human NALP3 (NP_004886).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q96P20 Related reagents
Entrez Gene
NLRP3 Related reagents
Regulatory
For research purposes only

Applications of NALP3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 2.5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P

Product Specific References

References for NALP3 antibody

  1. Liu, R.T. et al. (2014) Vinpocetine inhibits amyloid-beta induced activation of NF-κB, NLRP3 inflammasome and cytokine production in retinal pigment epithelial cells.
    Exp Eye Res. pii: S0014-4835(14)00185-7.