NAK antibody

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Rabbit anti NAK (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1610 C IC WB 0.1 mg
Rabbit anti NAK (C-Terminal) antibody recognizes human NF-kB-activating kinase (NAK), otherwise known as TANK-binding kinase 1 (TBK1). It is involved in the signalling cascade of NF-kB activation by directly phosphorylating and activating IKK Beta. It also mediates the production of IFN Beta and CCL5 and plays a key role in the innate immune response. In addition, it phosphorylates and activates the interferon regulatory factors -3 and -7, eventually leading to the development of a cellular antiviral state. It may also be involved in the induction of the antiviral interferon response. NAK is expressed ubiquitously, with higher expression in testis.

Rabbit anti NAK (C-Terminal) antibody does not cross-react with IKK Alpha, IKK Beta, IKK Gamma or IKK Epsilon.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human NAK was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to 17 amino acids near the carboxy terminus of human NAK.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9UHD2 Related reagents
Entrez Gene
TBK1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007249 I-kappaB kinase/NF-kappaB cascade
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0032480 negative regulation of type I interferon production
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0051219 phosphoprotein binding
Regulatory
For research purposes only

Applications of NAK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry
Immunohistology - Frozen 10ug/ml
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1610 detects a band of approximately 84kDa in MOLT4 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*