NADPH Oxidase 1 antibody

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Goat anti NADPH Oxidase 1

NADPH oxidase 1 antibody recognizes NADPH oxidase 1, also known as NOX-1, mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX or NOH-1. The antibody has been extensively validated for western blotting using whole cell lysates.

NADPH Oxidase 1 Antibody with Control Lysate

NADPH oxidase 1 antibody recognizes NADPH oxidase 1, also known as NOX-1, mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX or NOH-1. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00110 WB 100 µl
VPA00110KT WB 2 Westerns
With Lysate 400µg
Goat anti Human NADPH oxidase 1 antibody recognizes the NADPH oxidase 1, also known as NOX1, mitogenic oxidase 1, MOX1, NADH/NADPH mitogenic oxidase subunit P65-MOX or NOH1.

Encoded by the NOX1 gene, NADPH oxidase 1 is a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for NOX1 (provided by RefSeq, Nov 2012).

Goat anti Human NADPH Oxidase 1 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-DKATDIVTGLKQK, from the internal region of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9Y5S8 Related reagents
Entrez Gene
NOX1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0001525 angiogenesis
GO:0007165 signal transduction
GO:0005506 iron ion binding
GO:0005769 early endosome
GO:0022900 electron transport chain
GO:0006739 NADP metabolic process
GO:0006746 FADH2 metabolic process
GO:0006954 inflammatory response
GO:0009268 response to pH
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0016175 superoxide-generating NADPH oxidase activity
GO:0016477 cell migration
GO:0030054 cell junction
GO:0030171 voltage-gated proton channel activity
GO:0042554 superoxide anion generation
GO:0042743 hydrogen peroxide metabolic process
GO:0042995 cell projection
GO:0043020 NADPH oxidase complex
GO:0045726 positive regulation of integrin biosynthetic process
GO:0045730 respiratory burst
GO:0048365 Rac GTPase binding
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050660 flavin adenine dinucleotide binding
GO:0050661 NADP binding
GO:0051454 intracellular pH elevation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NADPH Oxidase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti NOX1 detects a band of approximately 72 kDa in K562 cell lysates.
Western Blotting
Anti NOX1 detects a band of approximately 72 kDa in K562 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB