N-Myc antibody

Rabbit anti N-Myc

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
N-Myc

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VPA00320
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Rabbit anti Human N-Myc antibody recognizes N-Myc, also known as v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog, class E basic helix-loop-helix protein 37, neuroblastoma MYC oncogene, neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene and oncogene NMYC, pp65/67.

The MYCN gene is a member of the MYC family and encodes N-Myc, a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of MYCN is associated with a variety of tumors, most notably neuroblastomas (provided by RefSeq, Jul 2008).

Rabbit anti Human N-Myc antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti N-Myc detects a band of approximately 50 kDa in HEK293 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 322-351 of human N-Myc
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P04198
Entrez Gene
MYCN
GO Terms
GO:0005515 protein binding
GO:0000785 chromatin
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0030528 transcription regulator activity
View more products with N-MYC specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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