Myosin Heavy Chain 9 antibody

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Rabbit anti Myosin Heavy Chain 9

Myosin heavy chain 9 antibody recognizes myosin-9, also known as cellular myosin heavy chain, type A, myosin-9, MYH9, non-muscle myosin heavy chain A, non-muscle myosin heavy chain IIa or non-muscle myosin heavy polypeptide 9.

Myosin Heavy Chain 9 Antibody with Control Lysate

Myosin heavy chain 9 antibody recognizes myosin-9, also known as cellular myosin heavy chain, type A, myosin-9, MYH9, non-muscle myosin heavy chain A, non-muscle myosin heavy chain IIa or non-muscle myosin heavy polypeptide 9.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00201 WB 100 µl
VPA00201KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human myosin heavy chain 9 antibody recognizes myosin heavy chain 9, also known as cellular myosin heavy chain, type A, myosin-9, non-muscle myosin heavy chain A, non-muscle myosin heavy chain IIa, non-muscle myosin heavy polypeptide 9 and nonmuscle myosin heavy chain II-A.

Encoded by the MYH9 gene, myosin heavy chain 9 (myosin-9) is a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). This protein contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness (provided by RefSeq, Dec 2011).

Rabbit anti Human myosin heavy chain 9 antibody detects a band of 227 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti myosin heavy chain 9 detects a band of approximately 227 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 1840-1867 amino acids from the C-terminal region of human myosin heavy chain 9
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P35579 Related reagents
Entrez Gene
MYH9 Related reagents
GO Terms
GO:0000146 microfilament motor activity
GO:0000910 cytokinesis
GO:0001525 angiogenesis
GO:0001725 stress fiber
GO:0001726 ruffle
GO:0001931 uropod
GO:0051015 actin filament binding
GO:0005829 cytosol
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005826 actomyosin contractile ring
GO:0006509 membrane protein ectodomain proteolysis
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0008360 regulation of cell shape
GO:0030048 actin filament-based movement
GO:0015031 protein transport
GO:0016459 myosin complex
GO:0030220 platelet formation
GO:0030224 monocyte differentiation
GO:0030898 actin-dependent ATPase activity
GO:0031532 actin cytoskeleton reorganization
GO:0032154 cleavage furrow
GO:0042803 protein homodimerization activity
GO:0043495 protein anchor
GO:0043531 ADP binding
GO:0043534 blood vessel endothelial cell migration
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Myosin Heavy Chain 9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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