Myeloperoxidase antibody

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Rabbit anti Myeloperoxidase

Myeloperoxidase antibody recognizes myeloperoxidase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00193 WB 100 µl
Rabbit anti Human myeloperoxidase antibody recognizes myeloperoxidase.

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils (provided by RefSeq, Jul 2008).

Rabbit anti Human myeloperoxidase antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti myeloperoxidase detects a band of approximately 60 kDa in HL-60 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Gentamicin Sulfate.
0.09% Sodium Azide (NaN3)
Immunogen
Human Leukocytes
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P05164 Related reagents
Entrez Gene
MPO Related reagents
GO Terms
GO:0003682 chromatin binding
GO:0006952 defense response
GO:0005764 lysosome
GO:0004601 peroxidase activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0030141 stored secretory granule
GO:0055114 oxidation-reduction process
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0020037 heme binding
GO:0034374 low-density lipoprotein particle remodeling
GO:0042744 hydrogen peroxide catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Myeloperoxidase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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