mTOR antibody

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Rabbit anti mTOR

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2498 WB 50 µl
Rabbit anti mTOR antibody recognizes mTOR also known as FRAP-1. mTOR is a serine/threonine kinase that functions as a cellular sensor for ATP and amino acids. mTOR is integral to cellular homeostasis and interacts with many signaling pathways involved in cancer.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mTOR was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Synthetic human mTOR peptide

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P42345 Related reagents
Entrez Gene
MTOR Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0016049 cell growth
GO:0005764 lysosome
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005942 phosphatidylinositol 3-kinase complex
GO:0007584 response to nutrient
GO:0008286 insulin receptor signaling pathway
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030163 protein catabolic process
GO:0031295 T cell costimulation
GO:0031929 TOR signaling cascade
GO:0031931 TORC1 complex
GO:0031932 TORC2 complex
GO:0043200 response to amino acid stimulus
GO:0046777 protein autophosphorylation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051219 phosphoprotein binding
Regulatory
For research purposes only

Applications of mTOR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti mTOR antibody detects a band of approximately 289 kDa in cell and tissue lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*