Rabbit anti mTOR antibody recognizes mTOR also known as FRAP-1. mTOR is a serine/threonine kinase that functions as a cellular sensor for ATP and amino acids. mTOR is integral to cellular homeostasis and interacts with many signaling pathways involved in cancer.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mTOR was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Synthetic human mTOR peptide
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein serine/threonine kinase activity
mitochondrial outer membrane
endoplasmic reticulum membrane
phosphatidylinositol 3-kinase complex
response to nutrient
insulin receptor signaling pathway
protein catabolic process
T cell costimulation
TOR signaling cascade
response to amino acid stimulus
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of mTOR antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti mTOR antibody detects a band of approximately 289 kDa in cell and tissue lysates under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available
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