MRE11 antibody

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Rabbit anti MRE11

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00590
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human MRE11 antibody recognizes the double-strand break repair protein MRE11, also known as AT-like disease, DNA recombination and repair protein, MRE11 homolog 1, MRE11 homolog A, endo/exonuclease Mre11, meiotic recombination 11 homolog 1 or meiotic recombination 11 homolog A.

MRE11A gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Mutations in the MRE11A gene causes Ataxia-telangiectasia-like disorder 1 (ATLD1), a rare disorder characterized by progressive cerebellar ataxia, dysarthria, abnormal eye movements, and absence of telangiectasia (Stewart et al. 1999). MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Rabbit anti Human MRE11 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MRE11 detects a band of approximately 81 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the middle region of human MRE11A

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P49959
Entrez Gene
MRE11A
GO Terms
GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity
GO:0000019 regulation of mitotic recombination
GO:0000724 double-strand break repair via homologous recombination
GO:0003690 double-stranded DNA binding
GO:0005654 nucleoplasm
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0007004 telomere maintenance via telomerase
GO:0007062 sister chromatid cohesion
GO:0007131 reciprocal meiotic recombination
GO:0008022 protein C-terminus binding
GO:0008408 3'-5' exonuclease activity
GO:0030145 manganese ion binding
GO:0030870 Mre11 complex
GO:0031954 positive regulation of protein autophosphorylation
GO:0032508 DNA duplex unwinding
GO:0032876 negative regulation of DNA endoreduplication
GO:0033674 positive regulation of kinase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of MRE11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml