MNAR antibody

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Rabbit anti MNAR

MNAR antibody recognizes proline-, glutamic acid- and leucine-rich protein 1, also known as PELP1, modulator of non-genomic activity of estrogen receptor or transcription factor HMX3. This antibody has been extensively validated for western blotting using whole cell lysates.

MNAR Antibody with Control Lysate

MNAR antibody recognizes proline-, glutamic acid- and leucine-rich protein 1, also known as PELP1, modulator of non-genomic activity of estrogen receptor or transcription factor HMX3. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00253 WB 100 µl
VPA00253KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human MNAR antibody recognizes modulator of non-genomic activity of estrogen receptor, otherwise known as proline-, glutamic acid-, and leucine-rich protein-1.

A product of the MNAR (PELP1) gene, this protein is a novel steroid co-activator of the estrogen receptor (ER) which plays a role in E2-mediated cell cycle progression through interaction with the pRb pathway. MNAR acts as a transcriptional corepressor for nuclear receptors and can interact with chromatin-modifying complex components CBP (CRE-binding protein), histone deacetylase 2 and MTA (metastasis-associated protein)1. Other properties of MNAR include interaction with PI3K, STAT3, and EGF, and SH2-binding which modulates the interaction of the ER to c-Src, leading to MAPK activation. Studies have shown the deregulation of MNAR in breast tumours and endometrial cancers.

Rabbit anti Human MNAR antibody detects a band of 160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 744-771 amino acids from the central region of human MNAR

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q8IZL8 Related reagents
Entrez Gene
PELP1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0071339 MLL1 complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MNAR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti MNAR detects a band of approximately 160 kDa in HEK293 cell lysates
Western Blotting
Anti MNAR detects a band of approximately 160 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB