MMP-3 antibody

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Rabbit anti MMP-3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00558
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human MMP-3 antibody recognizes matrix metalloproteinase 3 also known as proteoglycanase or transin-1.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The MMP3 gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3 (provided by RefSeq, Jul 2008).

Rabbit anti Human MMP-3 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MMP-3 detects a band of approximately 60 kDa in Ramos cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 30-59 of human MMP-3

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P08254
Entrez Gene
MMP3
GO Terms
GO:0005509 calcium ion binding
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0043231 intracellular membrane-bounded organelle
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of MMP-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml