MIP-3 Alpha antibody

Rabbit anti Human MIP-3 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MIP-3 Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP788
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
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Rabbit anti Human MIP-3 alpha antibody recognizes human MIP-3 alpha, (Macrophage Inflammatory Protein 3 alpha), otherwise known as CCL20, a 70 amino acid CC chemokine predominantly expressed by the liver, lymph nodes, appendix, spleen and pancreas and also by the thymus, small intestine, foetal lungs and PBLs.

MIP-3 alpha acts as a chemoattractant for memory T lymphocytes, immature dendritic cells and B lymphocytes, being the only CC chemokine known to bind to the G-protein linked 7-transmembrane receptor CCR6. Further properties of MIP-3 alpha include antimicrobial activity, inhibition of the in vitro proliferation of bone marrow immature myeloid progenitors and the stimulation of the growth and migration of pancreatic carcinoma cells.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add azide.
Antiserum Preparation
Antisera to human MIP-3 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human MIP-3 alpha.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 15.0 18.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Hieshima, K. et al. (1997) Molecular cloning of a novel human CC chemokine liver and activation-regulated chemokine (LARC) expressed in liver. Chemotactic activity for lymphocytes and gene localization on chromosome 2.
    J Biol Chem. 272 (9): 5846-53.
  2. Schutyser, E. et al. (2003) The CC chemokine CCL20 and its receptor CCR6.
    Cytokine Growth Factor Rev. 14 (5): 409-26.
  3. Kleeff, J. et al. (1999) Detection and localization of Mip-3alpha/LARC/Exodus, a macrophage proinflammatory chemokine, and its CCR6 receptor in human pancreatic cancer.
    Int J Cancer. 81 (4): 650-7.

Synonyms
CCL20
RRID
AB_808656
UniProt
P78556
Entrez Gene
CCL20
GO Terms
GO:0042742 defense response to bacterium
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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