MIP-1 Beta antibody

Rabbit anti Human MIP-1 Beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MIP-1 Beta

Product Code Applications Pack Size List Price Your Price Qty
AHP1043
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human MIP-1 beta antibody recognizes human MIP-1 beta, otherwise known as CCL4, a 69 amino acid member of the CC chemokine family, shown to signal through the chemokine receptors CCR1, CCR2 and CCR5.

MIP-1 beta is involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as monocytes, B cells, NK cells, neutrophils, dendritic cells and CD4+ lymphocytes.

The secretion of MIP-1 beta by CD8+ lymphocytes has been shown to act as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human MIP-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human MIP-1 beta.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Functional Assays 1.75 3.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for MIP-1 Beta antibody

  1. Cocchi, F. et al. (1995) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.
    Science. 270 (5243): 1811-5.

Further Reading

  1. Menten, P. et al. (2002) Macrophage inflammatory protein-1.
    Cytokine Growth Factor Rev. 13 (6): 455-81.

Synonyms
CCL4
RRID
AB_808652
UniProt
P13236
Entrez Gene
CCL4
GO Terms
GO:0009615 response to virus
GO:0007155 cell adhesion
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005615 extracellular space
GO:0006928 cellular component movement
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007163 establishment or maintenance of cell polarity
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0019079 viral genome replication
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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