MIP-1 Alpha antibody

100% Secure

Goat anti Human MIP-1 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP787 E WB 0.1 mg
Goat anti Human MIP-1 alpha antibody recognizes human MIP-1 alpha, otherwise known as CCL3, a 69 amino acid member of the CC chemokine family which plays a critical role in haematopoiesis and is shown to signal through the chemokine receptors CCR1, CCR4 and CCR5.

MIP-1 alpha is also involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as macrophages, NK cells, granulocytes, dendritic cells and CD8+ lymphocytes. The recruitment of inflammatory cells by MIP-1 alpha, has been linked with the pathogenesis of several diseases, including rheumatoid arthritis, H. pylori associated gastritis and bacterial meningitis.

The secretion of MIP-1 alpha by CD8+ lymphocytes acts as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry.

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human MIP-1 alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human MIP-1 alpha.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P10147 Related reagents
Entrez Gene
CCL3 Related reagents
GO Terms
GO:0007010 cytoskeleton organization
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006874 cellular calcium ion homeostasis
GO:0006887 exocytosis
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0042056 chemoattractant activity
GO:0045069 regulation of viral genome replication
Regulatory
For research purposes only

Applications of MIP-1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for MIP-1 Alpha antibody

  1. Cocchi, F. et al. (1995) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.
    Science. 270 (5243): 1811-5.

Further Reading

  1. Menten, P. et al. (2002) Macrophage inflammatory protein-1.
    Cytokine Growth Factor Rev. 13 (6): 455-81.