MIF antibody

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Goat anti MIF

MIF antibody recognizes macrophage migration inhibitory factor , also known as MIF, glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase or phenylpyruvate tautomerase. The antibody has been extensively validated for western blotting using whole cell lysates.

MIF Antibody with Control Lysate

MIF antibody recognizes macrophage migration inhibitory factor , also known as MIF, glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase or phenylpyruvate tautomerase. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00057 WB 100 µl
VPA00057KT WB 2 Westerns
With Lysate 400µg
Goat anti Human MIF antibody recognizes MIF, also known as macrophage migration inhibitory factor. In humans, the MIF protein is encoded by the MIF gene. This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Goat anti Human MIF antibody detects MIF as a single band of 13 kDa in a wide range of human cell line lysates.

Product Details

Target Species
Human
Western Blotting
Goat anti MIF detects a band of approximately 13 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-NAANVGWNNSTFA from the C terminus of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P14174 Related reagents
Entrez Gene
MIF Related reagents
GO Terms
GO:0001516 prostaglandin biosynthetic process
GO:0004167 dopachrome isomerase activity
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0010629 negative regulation of gene expression
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0030890 positive regulation of B cell proliferation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042056 chemoattractant activity
GO:0043030 regulation of macrophage activation
GO:0043066 negative regulation of apoptosis
GO:0043498 cell surface binding
GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045087 innate immune response
GO:0048146 positive regulation of fibroblast proliferation
GO:0050178 phenylpyruvate tautomerase activity
GO:0050715 positive regulation of cytokine secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0070207 protein homotrimerization
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071157 negative regulation of cell cycle arrest
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MIF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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