MIF antibody
Goat anti MIF
MIF antibody recognizes macrophage migration inhibitory factor , also known as MIF, glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase or phenylpyruvate tautomerase. The antibody has been extensively validated for western blotting using whole cell lysates.
MIF Antibody with Control Lysate
MIF antibody recognizes macrophage migration inhibitory factor , also known as MIF, glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase or phenylpyruvate tautomerase. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00057 | WB | 100 µl | ||||
| VPA00057KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human MIF antibody recognizes MIF, also known as macrophage migration inhibitory factor. In humans, the MIF protein is encoded by the MIF gene. This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Goat anti Human MIF antibody detects MIF as a single band of 13 kDa in a wide range of human cell line lysates.
Goat anti Human MIF antibody detects MIF as a single band of 13 kDa in a wide range of human cell line lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti MIF detects a band of approximately 13 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-NAANVGWNNSTFA from the C terminus of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P14174 Related reagents
- Entrez Gene
- MIF Related reagents
- GO Terms
- GO:0001516 prostaglandin biosynthetic process
- GO:0004167 dopachrome isomerase activity
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0006954 inflammatory response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0009986 cell surface
- GO:0010629 negative regulation of gene expression
- GO:0010739 positive regulation of protein kinase A signaling cascade
- GO:0030890 positive regulation of B cell proliferation
- GO:0033138 positive regulation of peptidyl-serine phosphorylation
- GO:0042056 chemoattractant activity
- GO:0043030 regulation of macrophage activation
- GO:0043066 negative regulation of apoptosis
- GO:0043498 cell surface binding
- GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
- GO:0045087 innate immune response
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0050178 phenylpyruvate tautomerase activity
- GO:0050715 positive regulation of cytokine secretion
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0070207 protein homotrimerization
- GO:0070374 positive regulation of ERK1 and ERK2 cascade
- GO:0071157 negative regulation of cell cycle arrest
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of MIF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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