MIF antibody

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Anti MIF Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human MIF antibody (**VPA00057**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 150 second exposure. Arrow points to MIF (molecular weight 13 kDa).

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Goat anti MIF

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: MIF

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00057 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human MIF antibody recognizes MIF, also known as macrophage migration inhibitory factor. In humans, the MIF protein is encoded by the MIF gene. This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Goat anti Human MIF antibody detects MIF as a single band of 13 kDa in a wide range of human cell line lysates.

Target Species: Human
Western Blotting: Goat anti MIF detects a band of approximately 13 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide with sequence C-NAANVGWNNSTFA from the C terminus of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P14174
Entrez Gene: MIF
GO Terms: GO:0001516 prostaglandin biosynthetic process; GO:0004167 dopachrome isomerase activity; GO:0005125 cytokine activity; GO:0005126 cytokine receptor binding; GO:0005615 extracellular space; GO:0005737 cytoplasm; GO:0006954 inflammatory response; GO:0007166 cell surface receptor linked signaling pathway; GO:0008283 cell proliferation; View More GO Terms; GO:0009986 cell surface; GO:0010629 negative regulation of gene expression; GO:0010739 positive regulation of protein kinase A signaling cascade; GO:0030890 positive regulation of B cell proliferation; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0042056 chemoattractant activity; GO:0043030 regulation of macrophage activation; GO:0043066 negative regulation of apoptosis; GO:0043498 cell surface binding; GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator; GO:0045087 innate immune response; GO:0048146 positive regulation of fibroblast proliferation; GO:0050178 phenylpyruvate tautomerase activity; GO:0050715 positive regulation of cytokine secretion; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0070207 protein homotrimerization; GO:0070374 positive regulation of ERK1 and ERK2 cascade; GO:0071157 negative regulation of cell cycle arrest