Goat anti Human MIB-1 antibody
recognizes human E3 ubiquitin-protein ligase MIB1, also known as Mind bomb homolog 1, DAPK-interacting protein 1 or Zinc finger ZZ type with ankyrin repeat domain protein 2, encoded by the MIB1 gene. E3 ubiquitin-protein ligase MIB1 is a 1006 amino acid ~130 kDa E3 ubiquitin-protein ligase which plays a role in the regulation of apoptosis through DAPK interaction, and also a key role in the positive regulation of the Notch signaling pathway.
MIB1 mediates the ubiquitination of the intracellular domain of the major Notch ligands known as Delta receptors, resulting in their endocytosis and degradation, and subsequently in the up-regulation of receptor activity and Notch pathway activation/signaling.MIB1 is highly expressed in the brain, spinal cord and ovaries, and elevated levels of both MIB1 and DAPK are found in the cytoplasm and microsomal fractions of epilepsy brain tissue.
Mutation of the MIB1 gene leads to the development of Left ventricular non-compaction 7 (LVNC7
), a disease caused by incomplete cardiac muscle development, characterized by a hypotrophic left ventricle (Luxán et al. 2013
), although it may on occasions be associated with other myocardial abnormalities (Ströllberger et al. 2002
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MIB1 antibody
|Immunohistology - Paraffin 1
- 1 This product requires antigen retrieval using heat treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2264 detects a band of approximately 135kDa in HEK293 lysate transiently expressing Human MIB1 tagged with myc and DYKDDDDK.