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MIB1 antibody

Goat anti Human MIB1

Product Type
Polyclonal Antibody
Polyclonal IgG

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
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Goat anti Human MIB-1 antibody recognizes human E3 ubiquitin-protein ligase MIB1, also known as Mind bomb homolog 1, DAPK-interacting protein 1 or Zinc finger ZZ type with ankyrin repeat domain protein 2, encoded by the MIB1 gene. E3 ubiquitin-protein ligase MIB1 is a 1006 amino acid ~130 kDa E3 ubiquitin-protein ligase which plays a role in the regulation of apoptosis through DAPK interaction, and also a key role in the positive regulation of the Notch signaling pathway.

MIB1 mediates the ubiquitination of the intracellular domain of the major Notch ligands known as Delta receptors, resulting in their endocytosis and degradation, and subsequently in the up-regulation of receptor activity and Notch pathway activation/signaling.MIB1 is highly expressed in the brain, spinal cord and ovaries, and elevated levels of both MIB1 and DAPK are found in the cytoplasm and microsomal fractions of epilepsy brain tissue.

Mutation of the MIB1 gene leads to the development of Left ventricular non-compaction 7 (LVNC7), a disease caused by incomplete cardiac muscle development, characterized by a hypotrophic left ventricle (Luxán et al. 2013), although it may on occasions be associated with other myocardial abnormalities (Ströllberger et al. 2002).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Bovine Expected from Sequence
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human MIB1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Synthetic peptide sequence C-DKDNTNVNADVQKLQ from the internal region of MIB1 (NP_065825.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1 This product requires antigen retrieval using heat treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2264 detects a band of approximately 135kDa in HEK293 lysate transiently expressing Human MIB1 tagged with myc and DYKDDDDK.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.

Mind Bomb Homolog 1
Entrez Gene
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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