MIB1 antibody
Goat anti Human MIB-1 antibody recognizes human E3 ubiquitin-protein ligase MIB1, also known as Mind bomb homolog 1, DAPK-interacting protein 1 or Zinc finger ZZ type with ankyrin repeat domain protein 2, encoded by the MIB1 gene. E3 ubiquitin-protein ligase MIB1 is a 1006 amino acid ~130 kDa E3 ubiquitin-protein ligase which plays a role in the regulation of apoptosis through DAPK interaction, and also a key role in the positive regulation of the Notch signaling pathway.
MIB1 mediates the ubiquitination of the intracellular domain of the major Notch ligands known as Delta receptors, resulting in their endocytosis and degradation, and subsequently in the up-regulation of receptor activity and Notch pathway activation/signaling.MIB1 is highly expressed in the brain, spinal cord and ovaries, and elevated levels of both MIB1 and DAPK are found in the cytoplasm and microsomal fractions of epilepsy brain tissue.
Mutation of the MIB1 gene leads to the development of Left ventricular non-compaction 7 (LVNC7), a disease caused by incomplete cardiac muscle development, characterized by a hypotrophic left ventricle (Luxán et al. 2013), although it may on occasions be associated with other myocardial abnormalities (Ströllberger et al. 2002).
MIB1 mediates the ubiquitination of the intracellular domain of the major Notch ligands known as Delta receptors, resulting in their endocytosis and degradation, and subsequently in the up-regulation of receptor activity and Notch pathway activation/signaling.MIB1 is highly expressed in the brain, spinal cord and ovaries, and elevated levels of both MIB1 and DAPK are found in the cytoplasm and microsomal fractions of epilepsy brain tissue.
Mutation of the MIB1 gene leads to the development of Left ventricular non-compaction 7 (LVNC7), a disease caused by incomplete cardiac muscle development, characterized by a hypotrophic left ventricle (Luxán et al. 2013), although it may on occasions be associated with other myocardial abnormalities (Ströllberger et al. 2002).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence Bovine Expected from Sequence Dog Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human MIB1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence C-DKDNTNVNADVQKLQ from the internal region of MIB1 (NP_065825.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of MIB1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/16000 | ||
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1 This product requires antigen retrieval using heat treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2264 detects a band of approximately 135kDa in HEK293 lysate transiently expressing Human MIB1 tagged with myc and DYKDDDDK.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
Further Reading
-
Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
Nat Rev Mol Cell Biol. 7 (9): 678-89.
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