MFN2 antibody

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Rabbit anti MFN2

Rabbit anti-human MFN2 antibody recognizes Mitofusin-2, also known as hyperplasia suppressor, mitochondrial assembly regulatory factor, mitofusin-2 or transmembrane GTPase MFN2. This antibody has been extensively validated for western blotting using whole cell lysates.

MFN2 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00448	WB	100 µl
VPA00448KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human MFN2 antibody recognizes MFN2, also known as hyperplasia suppressor, mitochondrial assembly regulatory factor or transmembrane GTPase MFN2.

The MFN2 gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in MFN2 gene cause Charcot-Marie-Tooth disease type 2A2 (CMT2A2), and hereditary motor and sensory neuropathy VI (HMSN6A), which are both disorders of the peripheral nervous system. Defects in MFN2 have also been associated with early-onset stroke (Chung et al. 2008). Two transcript variants encoding the same protein have been identified. (provided by RefSeq, Jul 2008).

Rabbit anti Human MFN2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti MFN2 detects a band of approximately 80 kDa in Ramos cell lysates.
Product Form: Purified IgG - liquid.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Preparation: 20μl Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide corresponding to amino acid 447-476 of human MFN2
Instructions For Use: Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition: 400μg Ramos lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: O95140 Related reagents
Entrez Gene: MFN2 Related reagents
GO Terms: GO:0003924 GTPase activity; GO:0007596 blood coagulation; GO:0016021 integral to membrane; GO:0005829 cytosol; GO:0005525 GTP binding; GO:0006626 protein targeting to mitochondrion; GO:0007006 mitochondrial membrane organization; GO:0008053 mitochondrial fusion; GO:0031306 intrinsic to mitochondrial outer membrane; GO:0031625 ubiquitin protein ligase binding; GO:0046580 negative regulation of Ras protein signal transduction; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0051646 mitochondrion localization
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only.

Applications of MFN2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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