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MEK 4 (pSer257/pThr261) antibody

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Anti MEK 4 (pSer257/pThr261) Antibody gallery image 1
Western blot analysis of extracts from HEK-293 cells untreated or treated with UV (for 15-30 minutes) using Rabbit anti MEK 4 (pSer257/pThr261) antibody (AHP2655).

Rabbit anti MEK 4 (pSer257/pThr261)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2655
Datasheet
WB
SDS
50 µl loader

Rabbit anti MEK 4 (pSer257/pThr261) antibody recognizes MAPK/ERK kinase 4 (MEK 4), also known as dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4 (MAPKK 4), stress-activated protein kinase kinase 1 and JNKK, when phosphorylated at serine 257/threonine 261.

MEK 4 is a dual specificity protein kinase and a member of the MAP kinase family. MEK 4 plays a key role in the MAP kinase and SAP/JNK signaling pathways.

MEK 4 is activated by phosphorylation at serine 257 and threonine 261 by MAP kinase kinase kinases (MAP3Ks). Downstream targets of activated MEK4 include JNK1, JNK2 and p38 MAPK.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to MEK 4 (pSer257/pThr261) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 257/threonine 261 of human MEK 4

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P45985
Entrez Gene
MAP2K4
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007254 JNK cascade
GO:0008063 Toll signaling pathway
GO:0008545 JUN kinase kinase activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of MEK 4 (pSer257/pThr261) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
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Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

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