Rabbit anti MEK 4 (pSer257/pThr261) antibody recognizes MAPK/ERK kinase 4 (MEK 4), also known as dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4 (MAPKK 4), stress-activated protein kinase kinase 1 and JNKK, when phosphorylated at serine 257/threonine 261.
MEK 4 is a dual specificity protein kinase and a member of the MAP kinase family. MEK 4 plays a key role in the MAP kinase and SAP/JNK signaling pathways.
MEK 4 is activated by phosphorylation at serine 257 and threonine 261 by MAP kinase kinase kinases (MAP3Ks). Downstream targets of activated MEK4 include JNK1, JNK2 and p38 MAPK.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MEK 4 (pSer257/pThr261) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 257/threonine 261 of human MEK 4
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
protein serine/threonine kinase activity
protein tyrosine kinase activity
Toll signaling pathway
JUN kinase kinase activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
innate immune response
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MEK 4 (pSer257/pThr261) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available