MEK 1 (pSer221) antibody

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Anti MEK 1 (pSer221) Antibody thumbnail image 2

Anti MEK 1 (pSer221) Antibody gallery image 1 — Western blot analysis of extracts from HeLa cells untreated or treated with EGF using Rabbit anti MEK 1 (pSer221) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

Anti MEK 1 (pSer221) Antibody gallery image 2 — Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti MEK 1 (pSer221) antibody

Rabbit anti MEK 1 (pSer221)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2649	P WB			50 µl

Rabbit anti MEK 1 (pSer221) antibody recognizes MAPK/ERK kinase 1 (MEK 1), also known as dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1 (MAPKK 1) and ERK activator kinase 1, when phosphorylated at serine 221.

MEK 1 is an integral component of the MAP kinase cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity.

MEK1 is phosphorylated at serine 217 and serine 221 by Raf kinases, increasing MEK1 activity (Matallanas et al. 2011) and subsequently leading to activation of ERK1/2.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to MEK 1 (pSer221) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide
50% Glycerol

Immunogen

Phospho specific-peptide corresponding to residues surrounding serine 221 of human MEK 1

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch

More Information

UniProt: Q02750 Related reagents
Entrez Gene: MAP2K1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0000186 activation of MAPKK activity; GO:0000187 activation of MAPK activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0004708 MAP kinase kinase activity; GO:0004713 protein tyrosine kinase activity; GO:0005524 ATP binding; GO:0007050 cell cycle arrest; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0008285 negative regulation of cell proliferation; GO:0008286 insulin receptor signaling pathway; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051403 stress-activated MAPK cascade; GO:0090398 cellular senescence
Regulatory: For research purposes only

Applications of MEK 1 (pSer221) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1/50	1/100
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*