MEK 1/2 (pSer218/pSer222) antibody

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Rabbit anti MEK 1/2 (pSer218/pSer222)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP904 WB* 0.1 ml
Rabbit anti MEK 1/2 (pSer218/pSer222) antibody recognizes MAPK/ERK kinase 1 (MEK 1) and MAPK/ERK kinase 2 (MEK 1), also known as MAP kinase kinase 1 (MAPKK 1) and MAP kinase kinase 2 (MAPKK 2) respectively, when phosphorylated at serine 218 and 222.

MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken Expected from Sequence
Bovine Expected from Sequence
Monkey Expected from Sequence
Rat Expected from Sequence
Zebrafish Expected from Sequence
Mouse
Dog Expected from Sequence
Xenopus Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated residues serine 218 and 222 within human MEK1 and 2.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q02750 Related reagents
P36507 Related reagents
Entrez Gene
MAP2K2 Related reagents
MAP2K1 Related reagents
GO Terms
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
GO:0070371 ERK1 and ERK2 cascade
GO:0005886 plasma membrane
GO:0004708 MAP kinase kinase activity
GO:0007050 cell cycle arrest
GO:0008285 negative regulation of cell proliferation
GO:0090398 cellular senescence
Regulatory
For research purposes only

Applications of MEK 1/2 (pSer218/pSer222) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP904 recognises a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

References for MEK 1/2 (pSer218/pSer222) antibody

  1. Salinas-Vera, Y.M. et al. (2018) Cooperative multi-targeting of signaling networks by angiomiR-204 inhibits vasculogenic mimicry in breast cancer cells.
    Cancer Lett. Jun 06 [Epub ahead of print].