MEK 1/2 (pSer218/pSer222) antibody
Rabbit anti MEK 1/2 (pSer218/pSer222) antibody recognizes MAPK/ERK kinase 1 (MEK 1) and MAPK/ERK kinase 2 (MEK 1), also known as MAP kinase kinase 1 (MAPKK 1) and MAP kinase kinase 2 (MAPKK 2) respectively, when phosphorylated at serine 218 and 222.
MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.
MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
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Target Species Cross Reactivity Chicken Expected from Sequence Bovine Expected from Sequence Monkey Expected from Sequence Rat Expected from Sequence Zebrafish Expected from Sequence Mouse Dog Expected from Sequence Xenopus Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated residues serine 218 and 222 within human MEK1 and 2.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q02750
- P36507
- Entrez Gene
- MAP2K2
- MAP2K1
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0004713 protein tyrosine kinase activity
- GO:0005524 ATP binding
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008063 Toll signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051403 stress-activated MAPK cascade
- GO:0070371 ERK1 and ERK2 cascade
- GO:0005886 plasma membrane
- GO:0004708 MAP kinase kinase activity
- GO:0007050 cell cycle arrest
- GO:0008285 negative regulation of cell proliferation
- GO:0090398 cellular senescence
- Regulatory
- For research purposes only
Applications of MEK 1/2 (pSer218/pSer222) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP904 recognises a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for MEK 1/2 (pSer218/pSer222) antibody
-
Salinas-vera, Y.M. et al. (2018) Cooperative multi-targeting of signaling networks by angiomiR-204 inhibits vasculogenic mimicry in breast cancer cells.
Cancer Lett. 432: 17-27.
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