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MEK 1/2 (pSer218/pSer222) antibody

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Anti MEK 1/2 (pSer218/pSer222) Antibody gallery image 1
Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45 kDa MEK1/2 was absent in control and stimulated by serum using Rabbit anti Human MEK 1/2 (pSer218/pSER222) antibody (AHP904).

Rabbit anti MEK 1/2 (pSer218/pSer222)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP904
Datasheet
WB
SDS
0.1 ml loader

Rabbit anti MEK 1/2 (pSer218/pSer222) antibody recognizes MAPK/ERK kinase 1 (MEK 1) and MAPK/ERK kinase 2 (MEK 1), also known as MAP kinase kinase 1 (MAPKK 1) and MAP kinase kinase 2 (MAPKK 2) respectively, when phosphorylated at serine 218 and 222.

MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken Expected from Sequence
Bovine Expected from Sequence
Monkey Expected from Sequence
Rat Expected from Sequence
Zebrafish Expected from Sequence
Mouse
Dog Expected from Sequence
Xenopus Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated residues serine 218 and 222 within human MEK1 and 2.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q02750
P36507
Entrez Gene
MAP2K2
MAP2K1
GO Terms
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
GO:0070371 ERK1 and ERK2 cascade
GO:0005886 plasma membrane
GO:0004708 MAP kinase kinase activity
GO:0007050 cell cycle arrest
GO:0008285 negative regulation of cell proliferation
GO:0090398 cellular senescence
Regulatory
For research purposes only

Applications of MEK 1/2 (pSer218/pSer222) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP904 recognises a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Product Specific References

References for MEK 1/2 (pSer218/pSer222) antibody

  1. Salinas-vera, Y.M. et al. (2018) Cooperative multi-targeting of signaling networks by angiomiR-204 inhibits vasculogenic mimicry in breast cancer cells.
    Cancer Lett. 432: 17-27.
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