Rabbit anti MEK 1/2 (pSer217/pSer221) antibody
recognizes MAPK/ERK kinase 1 (MEK 1) and MAPK/ERK kinase 2 (MEK 2), also known as MAP kinase kinase 1 (MAPKK 1) and MAP kinase kinase 2 (MAPKK 2) respectively, when phosphorylated at serine 217 and serine 221. MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulate cell growth and differentiation.
MEK1 and MEK2 are phosphorylated at serine 217 and serine 221 by Raf kinases, increasing the activity or MEK 1 and MEK 2 (Matallanas et al. 2011
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MEK 1/2 (pSer217/pSer221) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 217/221 of human MEK 1/2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPKK activity
activation of MAPK activity
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
protein serine/threonine kinase activity
protein tyrosine kinase activity
epidermal growth factor receptor signaling pathway
Ras protein signal transduction
Toll signaling pathway
insulin receptor signaling pathway
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
innate immune response
nerve growth factor receptor signaling pathway
stress-activated MAPK cascade
ERK1 and ERK2 cascade
MAP kinase kinase activity
cell cycle arrest
negative regulation of cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MEK 1/2 (pSer217/pSer221) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Matallanas, D. et al. (2011) Raf family kinases: old dogs have learned new tricks.
Genes Cancer. 2 (3): 232-60.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra