MEK 1/2 (pSer217/pSer221) antibody
Rabbit anti MEK 1/2 (pSer217/pSer221)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MEK 1/2 (pSer217/pSer221)
Antibody Quality and 
Unrivaled 
| Rabbit anti MEK 1/2 (pSer217/pSer221) antibody recognizes MAPK/ERK kinase 1 (MEK 1) and MAPK/ERK kinase 2 (MEK 2), also known as MAP kinase kinase 1 (MAPKK 1) and MAP kinase kinase 2 (MAPKK 2) respectively, when phosphorylated at serine 217 and serine 221. MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulate cell growth and differentiation. MEK1 and MEK2 are phosphorylated at serine 217 and serine 221 by Raf kinases, increasing the activity or MEK 1 and MEK 2 (Matallanas et al. 2011). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MEK 1/2 (pSer217/pSer221) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 217/221 of human MEK 1/2
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Matallanas, D. et al. (2011) Raf family kinases: old dogs have learned new tricks.
Genes Cancer. 2 (3): 232-60.
- UniProt
- Q02750
- P36507
- Entrez Gene
- MAP2K2
- MAP2K1
- GO Terms
- GO:0000186 activation of MAPKK activity
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0004713 protein tyrosine kinase activity
- View More GO Terms
- GO:0005524 ATP binding
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008063 Toll signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051403 stress-activated MAPK cascade
- GO:0070371 ERK1 and ERK2 cascade
- GO:0005886 plasma membrane
- GO:0004708 MAP kinase kinase activity
- GO:0007050 cell cycle arrest
- GO:0008285 negative regulation of cell proliferation
- GO:0090398 cellular senescence
View more products with MEK specificity
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