Rabbit anti MDM2 (pSer166) antibody
recognizes MDM2, also known as E3 ubiquitin-protein ligase Mdm2, E3 ubiquitin protein ligase, p53 E3 ubiquitin protein ligase homolog, p53-binding protein Mdm2, when phosphorylated at serine 166. MDM2 is an E3 ubiquitin-protein ligase and a member of the MDM2 protein family. MDM2 is an important negative regulator of the tumor suppressor p53. MDM2 is phosphorylated at serine 166 by Akt, leading to an increase in the E3 ubiquitin ligase activity of MDM2 (Malmlöf et al. 2007
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MDM2 (pSer166) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 166 of human MDM2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MDM2 (pSer166) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Malmlöf, M. et al. (2007) MEK-ERK-mediated phosphorylation of Mdm2 at Ser-166 in hepatocytes. Mdm2 is activated in response to inhibited Akt signaling.
J Biol Chem. 282 (4): 2288-96.
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