Sheep anti Human MDC1 antibody recognizes human Mediator of DNA damage checkpoint protein 1, also known as MDC1 or Nuclear factor with BRCT domains 1. MDC1 is a 2089 amino acid, ~250 kDa nuclear protein containing two BRCT domains and a single FHA domain. MDC1 is involved in checkpoint mediated cell cycle arest in response to DNA damage (UniProt: Q14676)
- Target Species
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human MDC1 (3838) were raised by repeated immunisations of sheep with highly purified antigen.
- Preservative Stabilisers
0.09% Sodium Azide
- Recombinant MDC1 BRCT domains (aa 1883 - 2089).
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
- Shelf Life
- 18 months from date of despatch.
- Q14676 Related reagents
- Entrez Gene
- MDC1 Related reagents
- For research purposes only
Applications of MDC1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- Western Blotting
- AHP799 detects 3 bands of approximately 250 kDa in HeLa and 293T cell lysates.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Rabbit anti Sheep IgG (H/L):Biotin||5184-2304||1.5 ml||C E F WB|
|Donkey anti Sheep/Goat IgG:DyLight®488||STAR88D488GA||0.1 mg||F IF|
|Donkey anti Sheep/Goat IgG:DyLight®549||STAR88D549GA||0.1 mg||F IF|
|Donkey anti Sheep/Goat IgG:DyLight®649||STAR88D649GA||0.1 mg||F IF|
|Donkey anti Sheep/Goat IgG:FITC||STAR88F||1 mg||C|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||1 ml||C E P WB|
Product Specific References
References for MDC1 antibody
Goldberg, M. et al. (2003) MDC1 is required for the intra-S-phase DNA damage checkpoint.
Nature. 421 (6926): 952-6.
Lou, Z. et al. (2003) MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.
Nature. 421 (6926): 957-61.
Shi, W. et al. (2008) Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent
J Biol Chem. 283: 31608-16.
Rai, R. et al (2008) Differential regulation of centrosome integrity by DNA damage response proteins.
Cell Cycle. 7: 2225-33.
Ichijima, Y. et al. (2011) MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells.
Genes Dev. 25: 959-71.
Sin, H.S. et al. (2012) Human postmeiotic sex chromatin and its impact on sex chromosome evolution.
Genome Res. 22: 827-36.
Nakada, S. et al. (2008) PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint.
EMBO Rep. 9: 1019-26.
Nakada, S. et al. (2010) Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
Nature. 466: 941-6.
Reichert, S. et al. (2011) Survivin inhibition and DNA double-strand break repair: A molecular mechanism to overcome radioresistance in glioblastoma
Radiother Oncol. 101: 51-8.
Capalbo, G. et al. (2010) Radiation-induced survivin nuclear accumulation is linked to DNA damage repair.
Int J Radiat Oncol Biol Phys. 77 (1): 226-34.
Cloutier, J.M. et al. (2016) Mammalian meiotic silencing exhibits sexually dimorphic features.
Chromosoma. 125 (2): 215-26.
Kato, Y. et al. (2015) FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.
Hum Mol Genet. 24 (18): 5234-49.
Stewart, G.S. et al. (2003) MDC1 is a mediator of the mammalian DNA damage checkpoint.
Nature. 421 (6926): 961-6.