MDC1 antibody

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Sheep anti Human MDC1 (aa1883-2089)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP799 IF WB 0.1 ml
Sheep anti Human MDC1 antibody recognizes human Mediator of DNA damage checkpoint protein 1, also known as MDC1 or Nuclear factor with BRCT domains 1. MDC1 is a 2089 amino acid, ~250 kDa nuclear protein containing two BRCT domains and a single FHA domain. MDC1 is involved in checkpoint mediated cell cycle arest in response to DNA damage (UniProt: Q14676)

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human MDC1 (3838) were raised by repeated immunisations of sheep with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant MDC1 BRCT domains (aa 1883 - 2089).

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q14676 Related reagents
Entrez Gene
MDC1 Related reagents
Regulatory
For research purposes only

Applications of MDC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/500
Western Blotting 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Western Blotting
AHP799 detects 3 bands of approximately 250 kDa in HeLa and 293T cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

References for MDC1 antibody

  1. Goldberg, M. et al. (2003) MDC1 is required for the intra-S-phase DNA damage checkpoint.
    Nature. 421 (6926): 952-6.
  2. Lou, Z. et al. (2003) MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.
    Nature. 421 (6926): 957-61.
  3. Shi, W. et al. (2008) Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation.
    J Biol Chem. 283: 31608-16.
  4. Rai, R. et al (2008) Differential regulation of centrosome integrity by DNA damage response proteins.
    Cell Cycle. 7: 2225-33.
  5. Ichijima, Y. et al. (2011) MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells.
    Genes Dev. 25: 959-71.
  6. Sin, H.S. et al. (2012) Human postmeiotic sex chromatin and its impact on sex chromosome evolution.
    Genome Res. 22: 827-36.
  7. Nakada, S. et al. (2008) PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint.
    EMBO Rep. 9: 1019-26.
  8. Nakada, S. et al. (2010) Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
    Nature. 466: 941-6.
  9. Reichert, S. et al. (2011) Survivin inhibition and DNA double-strand break repair: A molecular mechanism to overcome radioresistance in glioblastoma
    Radiother Oncol. 101: 51-8.
  10. Capalbo, G. et al. (2010) Radiation-induced survivin nuclear accumulation is linked to DNA damage repair.
    Int J Radiat Oncol Biol Phys. 77 (1): 226-34.
  11. Cloutier, J.M. et al. (2016) Mammalian meiotic silencing exhibits sexually dimorphic features.
    Chromosoma. 125 (2): 215-26.
  12. Kato, Y. et al. (2015) FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.
    Hum Mol Genet. 24 (18): 5234-49.

Further Reading

  1. Stewart, G.S. et al. (2003) MDC1 is a mediator of the mammalian DNA damage checkpoint.
    Nature. 421 (6926): 961-6.