Sheep anti Human MDC1 antibody
recognizes human Mediator of DNA damage checkpoint protein 1, also known as MDC1 or Nuclear factor with BRCT domains 1. MDC1 is a 2089 amino acid, ~250 kDa nuclear protein containing two BRCT
domains and a single FHA
domain. MDC1 is involved in checkpoint mediated cell cycle arest in response to DNA damage (UniProt: Q14676
- Target Species
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human MDC1 (3838) were raised by repeated immunisations of sheep with highly purified antigen.
- Preservative Stabilisers
- Recombinant MDC1 BRCT domains (aa 1883 - 2089).
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
- 18 months from date of despatch.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MDC1 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- Western Blotting
- AHP799 detects 3 bands of approximately 250 kDa in HeLa and 293T cell lysates.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for MDC1 antibody
Goldberg, M. et al. (2003) MDC1 is required for the intra-S-phase DNA damage checkpoint.
Nature. 421 (6926): 952-6.
Lou, Z. et al. (2003) MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.
Nature. 421 (6926): 957-61.
Shi, W. et al. (2008) Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent
J Biol Chem. 283: 31608-16.
Rai, R. et al (2008) Differential regulation of centrosome integrity by DNA damage response proteins.
Cell Cycle. 7: 2225-33.
Ichijima, Y. et al. (2011) MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells.
Genes Dev. 25: 959-71.
Sin, H.S. et al. (2012) Human postmeiotic sex chromatin and its impact on sex chromosome evolution.
Genome Res. 22: 827-36.
Nakada, S. et al. (2008) PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint.
EMBO Rep. 9: 1019-26.
Nakada, S. et al. (2010) Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
Nature. 466: 941-6.
Reichert, S. et al. (2011) Survivin inhibition and DNA double-strand break repair: A molecular mechanism to overcome radioresistance in glioblastoma
Radiother Oncol. 101: 51-8.
Capalbo, G. et al. (2010) Radiation-induced survivin nuclear accumulation is linked to DNA damage repair.
Int J Radiat Oncol Biol Phys. 77 (1): 226-34.
Cloutier, J.M. et al. (2016) Mammalian meiotic silencing exhibits sexually dimorphic features.
Chromosoma. 125 (2): 215-26.
Kato, Y. et al. (2015) FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis.
Hum Mol Genet. 24 (18): 5234-49.
Stewart, G.S. et al. (2003) MDC1 is a mediator of the mammalian DNA damage checkpoint.
Nature. 421 (6926): 961-6.