MD-2 antibody
Rabbit anti MD-2 antibody recognizes MD-2, a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins which include Toll like receptors. TLR4 requires the binding of MD-2 to its extracellular region for best response to bacterial LPS. MD-2 also enhances TLR4-dependent activation of NF-kappa-beta and cooperates with TLR2 in the response to cell wall components from bacteria.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to MD-2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to amino acids near the middle of human MD-2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y6Y9
- Q56DL5
- Q9JHF9
- Entrez Gene
- Ly96
- LY96
- GO Terms
- GO:0005515 protein binding
- GO:0001875 lipopolysaccharide receptor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0032497 detection of lipopolysaccharide
- GO:0045087 innate immune response
- GO:0005886 plasma membrane
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0006968 cellular defense response
- GO:0007249 I-kappaB kinase/NF-kappaB cascade
- GO:0008063 Toll signaling pathway
- GO:0015026 coreceptor activity
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0046696 lipopolysaccharide receptor complex
- Regulatory
- For research purposes only
Applications of MD-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 2ug/ml | ||
| Western Blotting | 0.5 | 2.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Rat spleen.
- Western Blotting
- AHP1717T detects a band of approximately 26kDa in mouse spleen cell lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Fluorescent Spectraviewer
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- Select combined or multi-laser view to visualize the spectra
