MD-2 antibody

Rabbit anti MD-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MD-2

Product Code Applications Pack Size List Price Your Price Qty
AHP1717T
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SDS Safety Datasheet SDS
P * WB 50 µg loader
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Rabbit anti MD-2 antibody recognizes MD-2, a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins which include Toll like receptors. TLR4 requires the binding of MD-2 to its extracellular region for best response to bacterial LPS. MD-2 also enhances TLR4-dependent activation of NF-kappa-beta and cooperates with TLR2 in the response to cell wall components from bacteria.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to MD-2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the middle of human MD-2.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2ug/ml
Western Blotting 0.5 2.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Rat spleen.
Western Blotting
AHP1717T detects a band of approximately 26kDa in mouse spleen cell lysate

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RRID
AB_2138091
UniProt
Q9Y6Y9
Q56DL5
Q9JHF9
Entrez Gene
Ly96
LY96
GO Terms
GO:0005515 protein binding
GO:0001875 lipopolysaccharide receptor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0032497 detection of lipopolysaccharide
GO:0045087 innate immune response
GO:0005886 plasma membrane
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006968 cellular defense response
GO:0007249 I-kappaB kinase/NF-kappaB cascade
GO:0008063 Toll signaling pathway
GO:0015026 coreceptor activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0046696 lipopolysaccharide receptor complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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