MCT2 antibody

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Goat anti Human MCT2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP728 WB 0.1 mg
Goat anti Human MCT2 antibody recognizes human monocarboxylate transporter 2, also known as MCT2 or Solute carrier family 16 member 7. MCT2 is a 478 amino acid ~52 kDa multi-pass transmembrane transporter molecule involved in the transport of pyruvate and lactate across cell membranes.

Northern blot analysis reveals expression of a larger transcript in most normal tissues compared to that which predominates in many cell lines (Lin et al. 1998) and this may be reflected in the slightly lower molecular weight band detected in lung carcinoma cell lysate by this antibody in western blots.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human MCT2 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Synthetic peptide SNAQSVTSERETNI derived from C terminus of human MCT2.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O60669 Related reagents
Entrez Gene
SLC16A7 Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005477 pyruvate secondary active transmembrane transporter activity
GO:0005624 membrane fraction
GO:0015293 symporter activity
GO:0015355 secondary active monocarboxylate transmembrane transporter activity
Regulatory
For research purposes only

Applications of MCT2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP728 detects bands of approximately 40 kDa and 60-70 kD in Raji cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

Further Reading

  1. Lin, R.Y. et al. (1998) Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter.
    J Biol Chem. 273 (44): 28959-65.