Rabbit anti Human MCP-2 antibody recognizes human MCP-2 (Monocyte Chemoattractant Protein 2), otherwise known as CCL8, a 76 amino acid CC chemokine originally isolated from the human osteosarcoma cell line MG-63, which is expressed at highest levels in peripheral blood cells and the small intestine.
MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, due to its ability to bind to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-Terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional C-C chemokine inhibitor, which may be important during inflammatory responses.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human MCP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant human MCP-2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
response to virus
signal transducer activity
calcium ion transport
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MCP-2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
VanDamme, J. et al. (1992) Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family.
J Exp Med. 176 (1): 59-65.