Rabbit anti Human MCP-2 antibody recognizes human MCP-2 (Monocyte Chemoattractant Protein 2), otherwise known as CCL8, a 76 amino acid CC chemokine originally isolated from the human osteosarcoma cell line MG-63, which is expressed at highest levels in peripheral blood cells and the small intestine.
MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, due to its ability to bind to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-Terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional C-C chemokine inhibitor, which may be important during inflammatory responses.
- Target Species
- Product Form
- Purified IgG - lyophilized
- Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human MCP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant human MCP-2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to virus
signal transducer activity
calcium ion transport
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MCP-2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
VanDamme, J. et al. (1992) Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family.
J Exp Med. 176 (1): 59-65.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra