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MCP-1 antibody

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Anti Human MCP-1 Antibody thumbnail image 1 Anti Human MCP-1 Antibody thumbnail image 2 Anti Human MCP-1 Antibody thumbnail image 3
Anti Human MCP-1 Antibody gallery image 1
Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human MCP-1 (AHP784) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Anti Human MCP-1 Antibody gallery image 2
Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human MCP-1 (AHP784 )following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Anti Human MCP-1 Antibody gallery image 3
Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 (MCA2486) as a capture reagent and biotinylated Rabbit anti Human MCP-1 (AHP784B) as a detection reagent with recombinant human MCP-1 (PHP061) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are included undiluted

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Rabbit anti Human MCP-1:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
MCP-1

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Product Code Applications Pack Size List Price Your Price Qty
AHP784B
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Rabbit anti Human MCP-1 antibody recognizes human MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, a 76 amino acid CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2.

The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. MCP-1 is linked with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response.

Rabbit anti Human MCP-1 antibody has been successfully used as a detection reagent in the development of a sensitive ELISA assay for the evaluation of MCP-1 expression in conjunction with Mouse anti Human MCP-1, clone 2.2-4A4-1A11 (MCA2486) and recombinant human MCP-1 (PHP061) as a calibration reagent.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human MCP-1 were raised by repeated immunisations of rabbitts with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Carrier Free
Yes
Immunogen
Recombinant human MCP-1 ( PHP061).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with either AHP784 or MCA2486 as the capture reagent and PHP061 as the standard
Western Blotting
This product may be used under either reducing or non-reducing conditions with PHP061 as the positive control.

Further Reading

  1. Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination.
    J Neuroinflammation. 2 (1): 7.

Synonyms
CCL2
RRID
AB_2071555
UniProt
P13500
Entrez Gene
CCL2
GO Terms
GO:0002548 monocyte chemotaxis
GO:0007155 cell adhesion
GO:0004672 protein kinase activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0006874 cellular calcium ion homeostasis
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006959 humoral immune response
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
GO:0007259 JAK-STAT cascade
GO:0008009 chemokine activity
GO:0019079 viral genome replication
GO:0031727 CCR2 chemokine receptor binding
GO:0048246 macrophage chemotaxis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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