MCP-1 antibody
Filter by Application:
P E ResetRabbit anti Human MCP-1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- MCP-1
Antibody Quality and 
Unrivaled 
| Rabbit anti Human MCP-1 antibody recognizes human MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, a 76 amino acid CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2. The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. MCP-1 is linked with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response. Rabbit anti Human MCP-1 antibody has been successfully used as a detection reagent in the development of a sensitive ELISA assay for the evaluation of MCP-1 expression in conjunction with Mouse anti Human MCP-1, clone 2.2-4A4-1A11 (MCA2486) and recombinant human MCP-1 (PHP061) as a calibration reagent. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human MCP-1 ( PHP061).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Immunohistology - Paraffin 1 | |
2.5ug/ml | |
| Western Blotting | |
0.1ug/ml | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using apropriate negative/positive controls.
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP784B as the detection reagent and PHP061 as the standard.
- Histology Positive Control Tissue
- Human Breast Invasive Ductal Carcinoma
- Western Blotting
- This product may be used under either reducing or non-reducing conditions with PHP061 as the positive control.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
 |
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Further Reading
-
Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination.
J Neuroinflammation. 2 (1): 7.
- Synonyms
- CCL2
- RRID
- AB_2275395
- UniProt
- P13500
- Entrez Gene
- CCL2
- GO Terms
- GO:0002548 monocyte chemotaxis
- GO:0007155 cell adhesion
- GO:0004672 protein kinase activity
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0006874 cellular calcium ion homeostasis
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- View More GO Terms
- GO:0006959 humoral immune response
- GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
- GO:0007259 JAK-STAT cascade
- GO:0008009 chemokine activity
- GO:0019079 viral genome replication
- GO:0031727 CCR2 chemokine receptor binding
- GO:0048246 macrophage chemotaxis
View more products with MCP-1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up